NR0B1 抗体 (AA 359-408)
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- 抗原 See all NR0B1 抗体
- NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))
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抗原表位
- AA 359-408
- 适用
- 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠, 兔, 猴, 斑马鱼, 小鸡, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NR0B1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Human NR0B1 / DAX-1
- 交叉反应
- 小鸡, 犬, 小鼠, 猪, 大鼠
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Zebrafish (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide located between aa359-408 of human NR0B1 (Q9BG96, NP_000466). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard (100%), Galago, Hamster, Xenopus, Seabass, Salmon, Stickleback, Medaka (92%), Bat (91%), Zebrafish (85%), Catfish (81%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product NR0B1 Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- PBS, 0.09 % sodium azide, 2 % sucrose
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))
- 别名
- NR0B1 / DAX1 (NR0B1 产品)
- 别名
- DAX antibody, NR0B1 antibody, dax1 antibody, AHC antibody, AHCH antibody, AHX antibody, DAX-1 antibody, DAX1 antibody, DSS antibody, GTD antibody, HHG antibody, NROB1 antibody, SRXY2 antibody, Ahc antibody, Ahch antibody, Dax1 antibody, nuclear receptor subfamily 0 group B member 1 antibody, nuclear receptor subfamily 0, group B, member 1 antibody, nuclear receptor subfamily 0 group B member 1 L homeolog antibody, NR0B1 antibody, nr0b1 antibody, nr0b1.L antibody, Nr0b1 antibody
- 背景
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Name/Gene ID: NR0B1
Subfamily: NR0 Knirps-like
Family: NHR
Synonyms: NR0B1, AHX, AHC, AHCH, DAX1, DSS, DAX 1, DAX-1, Adrenal hypoplasia congenita, Dosage-sensitive sex reversal, GTD, HHG, Nuclear hormone receptor, NROB1, Nuclear receptor DAX-1, SRXY2, Nuclear receptor DAX1, Dax-1 receptor - 基因ID
- 190
- NCBI登录号
- NP_000466
- UniProt
- P51843
- 途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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