HNF4A 抗体 (N-Term)
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- 抗原 See all HNF4A 抗体
- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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抗原表位
- N-Term
- 适用
- 人, 小鼠, 大鼠, Cow, Pig, 犬, 猴, 马, 兔, 豚鼠, 绵羊, Bat, Hamster, 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HNF4A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human HNF4A / HNF4, immunogenic peptide sequence is present in isoforms 1, 2, 3, and 4.
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Zebrafish (92%) Xenopus (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide from N-Terminus of human HNF4A (P41235, NP_000448). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard (100%), Catfish, Stickleback, Zebrafish (92%), Xenopus (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HNF4A Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
- 别名
- HNF4A / HNF4 (HNF4A 产品)
- 背景
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Name/Gene ID: HNF4A
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR
Synonyms: HNF4A, Hepatic nuclear factor 4 alpha, HNF4, HNF4a8, NR2A21, TCF, TCF-14, TCF14, MODY1, Transcription factor 14, Transcription factor HNF-4, HNF-4-alpha, HNF4 alpha, HNF4a7, HNF4a9, HNF4alpha, HNF4alpha10/11/12, MODY, NR2A1 - 基因ID
- 3172
- NCBI登录号
- NP_000448
- UniProt
- P41235
- 途径
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
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