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BRCA2 抗体 (AA 21-130)

Name: BRCA2 抗体 (AA 21-130)
SKU: ABIN673434
Availability: OutOfStock
Rating: 5 (1 reviews)

BRCA2 适用: 人 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Breast Cancer 2, Early Onset (BRCA2) (AA 21-130) antibody (ABIN673434) (BRCA2 抗体 (AA 21-130))

Image provided by the Independent Validation Program badge 29807. (BRCA2 抗体 (AA 21-130))

Formalin-fixed and paraffin embedded human breast carcinoma labeled Anti-BRCA2 Polyclonal Antibody, Unconjugated (ABIN673434) at 1:200, followed by conjugation to the secondary antibody and DAB staining (BRCA2 抗体 (AA 21-130))

IHC

3 images

(1 validation)

产品编号 ABIN673434

发货至: 中国

Datasheet as PDF

抗原 See all BRCA2 抗体

BRCA2 (Breast Cancer 2, Early Onset (BRCA2))
抗原表位
12

7

6

4

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 21-130
适用
43

23

20

4

2

1

1

1
人
宿主
42

7
兔
Compatible Secondaries
克隆类型
41

8
多克隆
标记
32

3

2

2

2

2

2

2

1

1
This BRCA2 antibody is un-conjugated
应用范围
24

14

10

6

5

4

2

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人

预测反应

Mouse,Rat,Dog,Cow,Horse,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human BRCA2

亚型

IgG
anti-Breast Cancer 2, Early Onset (BRCA2) antibody
BRCA2 适用: 人, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2990-3232) antibody
BRCA2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 3319-3418) antibody
BRCA2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D12 unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 1-300) antibody
BRCA2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2913-3151) antibody
BRCA2 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2308-2537) antibody
BRCA2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 2737-2874) antibody
BRCA2 适用: 斑马鱼 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (pSer3291) antibody
BRCA2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) (AA 251-495) antibody
BRCA2 适用: 人 WB 宿主: 小鼠 Monoclonal 1643CT739-91-87 unconjugated

anti-Breast Cancer 2, Early Onset (BRCA2) antibody
BRCA2 适用: 人 WB, IF, IC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029807 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029807

日期

2014.08.26

抗原

Lot Number

101020

Method validated

Western Blotting

Positive Control

MCF7 cells

Negative Control

[CAPAN1 cells] (http://www.pnas.org/content/98/15/8644.full.pdf)

Notes

A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.

Validation Images

Figure 1: Western Blot for BRCA2. Arrowhead indicates the expected molecular weight of ~384 kDa.
Full Methods
Primary Antibody

Antigen: Breast Cancer 2, Early Onset (BRCA2)
Catalog number: ABIN673434
Lot number: 101020
Antibody Dilution: 1:200

Secondary Antibody

Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Lot number: L170-6515
Antibody Dilution: 1:10,000

Full Protocol

1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 10 hours at 4°C.
8. The membrane was rinsed in TBST thrice for 5 minutes each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
11. The membrane was rinsed in TBS twice for 30 seconds each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 5 minutes.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
15. The membrane was washed in TBST 2 times for 10 minutes each.
16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.

Experimental Notes

- No experimental challenges noted.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

别名

BRCA2 (BRCA2 产品)

别名

BRCC2 antibody, BROVCA2 antibody, FACD antibody, FAD antibody, FAD1 antibody, FANCB antibody, FANCD antibody, FANCD1 antibody, GLM3 antibody, PNCA2 antibody, 30169 antibody, BcDNA:SD25109 antibody, CG13583 antibody, CG13584 antibody, CG30169 antibody, Dmel\\CG30169 antibody, brca2 antibody, dmbrca2 antibody, fancd1 antibody, BRCA2 antibody, brcc2 antibody, brovca2 antibody, facd antibody, fad antibody, fad1 antibody, fancb antibody, AI256696 antibody, AW045498 antibody, Fancd1 antibody, RAB163 antibody, BRCA2, DNA repair associated antibody, Breast cancer 2, early onset homolog antibody, breast cancer 2, early onset antibody, BRCA2 antibody, Brca2 antibody, brca2 antibody, LOC584780 antibody

背景

Synonyms: FAD, FACD, FAD1, GLM3, BRCC2, FANCD, PNCA2, FANCD1, BROVCA2, Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein, BRCA2
Background: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).

基因ID

675

UniProt

P51587

途径

DNA Damage Repair, M Phase, Maintenance of Protein Location