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Recombinant Rpb1 CTD 抗体 (pTyr1)

适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
产品编号 ABIN6731014
发货至: 中国
  • 抗原 See all Rpb1 CTD products
    Rpb1 CTD (RNA Polymerase II CTD)
    抗体类型
    Recombinant Antibody
    抗原表位
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    pTyr1
    适用
    • 23
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 11
    • 9
    • 3
    小鼠
    克隆类型
    • 14
    • 9
    单克隆
    标记
    • 23
    This Rpb1 CTD antibody is un-conjugated
    应用范围
    • 23
    • 17
    • 15
    • 10
    • 7
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), ELISA
    品牌
    AbFlex®
    纯化方法
    Protein A Chromatography
    免疫原
    This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
    亚型
    IgG2a
  • 应用备注

    Validated Applications:
    IF: 0.5 - 2 µg/ml
    Bead-based ELISA: 0.5 - 10 µg/ml
    WB: 1 - 2 μg/mL

    For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required.
    说明

    AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • 抗原
    Rpb1 CTD (RNA Polymerase II CTD)
    别名
    RNA Pol II CTD (Rpb1 CTD 产品)
    分子量
    210 kDa
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