Recombinant Rpb1 CTD 抗体 (pTyr1)
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- 抗原 See all Rpb1 CTD products
- Rpb1 CTD (RNA Polymerase II CTD)
- 抗体类型
- Recombinant Antibody
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抗原表位
- pTyr1
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Rpb1 CTD antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- 品牌
- AbFlex®
- 纯化方法
- Protein A Chromatography
- 免疫原
- This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
- 亚型
- IgG2a
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- 应用备注
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Validated Applications:
IF: 0.5 - 2 µg/ml
Bead-based ELISA: 0.5 - 10 µg/ml
WB: 1 - 2 μg/mL
For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required. - 说明
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AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- Rpb1 CTD (RNA Polymerase II CTD)
- 别名
- RNA Pol II CTD (Rpb1 CTD 产品)
- 分子量
- 210 kDa
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