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CDKN1B 抗体 (AA 150-198)

Name: CDKN1B 抗体 (AA 150-198)
SKU: ABIN672621
Availability: OutOfStock

CDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Dnmt3a3 overexpression decreases fat accumulation in differentiating preadipocytes. (CDKN1B 抗体 (AA 150-198))

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated (ABIN672621) at 1:200, followed by conjugation to the secondary antibody and DAB staining (CDKN1B 抗体 (AA 150-198))

IHC

Mouse lung lysates probed with CDKN1B Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (CDKN1B 抗体 (AA 150-198))

MCF-7 cells were stained with CDKN1B Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. (CDKN1B 抗体 (AA 150-198))

IF (cc)

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (CDKN1B 抗体 (AA 150-198))

IHC (p)

RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (CDKN1B 抗体 (AA 150-198))

FACS

Expression of Dnmt3a spliced variants in adipose tissues and during the time-course analysis of preadipocyte differentiation. (CDKN1B 抗体 (AA 150-198))

7 images

(5 references)

产品编号 ABIN672621

发货至: 中国

Datasheet as PDF

抗原 See all CDKN1B 抗体

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
抗原表位
26

20

20

18

15

10

9

8

8

8

8

8

7

7

6

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 150-198
适用
246

114

102

26

6

6

3

3

3

3

2

1

1
人, 小鼠, 大鼠
宿主
212

47

2

1

1
兔
Compatible Secondaries
克隆类型
203

60
多克隆
标记
135

18

16

13

13

11

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This CDKN1B antibody is un-conjugated
应用范围
183

115

62

59

49

41

34

26

22

15

15

11

7

6

3

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鸡, 人, 小鼠, 大鼠

预测反应

Dog,Sheep,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CDKN1B

亚型

IgG
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4B4-E6 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
KO Validated CDKN1B 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal SX53G8 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 1G2 unconjugated Recombinant Antibody

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B 适用: 小鼠 WB, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal DCS-72-F6 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody
CDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-100) antibody
CDKN1B 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 2-197) antibody
CDKN1B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Abdalla, Li, Nie: "A Novel DNA Methyltransferase Dnmt3a3 Splice Variant Represses Preadipocyte Proliferation and Differentiation." in: Frontiers in genetics, Vol. 11, pp. 115, (2020) (PubMed).

Guo, Xu, Ren, Zhang, Zhang, Duan, Zhou, Sun: "Endosulfan induces apoptosis by activating the negative regulation pathway of cell cycle and death receptor pathway in spermatogenic cells." in: Toxicology research, Vol. 6, Issue 2, pp. 223-231, (2017) (PubMed).

Akizuki, Shimobaba, Matsunaga, Endo, Ikari: "Claudin-5, -7, and -18 suppress proliferation mediated by inhibition of phosphorylation of Akt in human lung squamous cell carcinoma." in: Biochimica et biophysica acta, Vol. 1864, Issue 2, pp. 293-302, (2016) (PubMed).

Gao, Tang, Ding, Wang, Qi, Wu, Guo: "Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-?B/c-Myc Pathway in Pancreatic ? cells." in: Molecular medicine (Cambridge, Mass.), Vol. 21, Issue 1, pp. 154-66, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).
抗原

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

别名

CDKN1B (CDKN1B 产品)

背景

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, CDKN1B
Background: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

基因ID

1027

UniProt

P46527

途径

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy