GST 抗体 (DyLight 680)
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- 抗原 See all GST 抗体
- GST (Glutathione S Transferase (GST))
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适用
- 标记
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GST antibody is conjugated to DyLight 680
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应用范围
- FLISA, Fluorescence Microscopy (FM)
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
- 免疫原
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Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type: Native Protein
- 亚型
- IgG
- Labeling Ratio
- 2.9
- Top Product
- Discover our top product GST Primary Antibody
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- 应用备注
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Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- 抗原
- GST (Glutathione S Transferase (GST))
- 别名
- GST (GST 产品)
- 别名
- GST antibody, GST 13-13 antibody, GST13 antibody, GST13-13 antibody, GSTK1-1 antibody, hGSTK1 antibody, 1500002K10Rik antibody, Gst antibody, Ptgds2 antibody, glutathione S-transferase kappa 1 antibody, microsomal glutathione S-transferase 1 antibody, hematopoietic prostaglandin D synthase antibody, GSTK1 antibody, Mgst1 antibody, Hpgds antibody
- 背景
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Synonyms: rabbit anti-GST antibody DyLight™680 conjugation, DyLight™680 conjugated rabbit anti-GST antibody, Glutathione-S-Transferase
Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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