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GFP 抗体 (DyLight 488)

Name: GFP 抗体 (DyLight 488)
SKU: ABIN6720701
Availability: OutOfStock

Rockland 600-141-215 GFP 适用: Aequorea victoria WB, ELISA, FM, DB, FLISA 宿主: 山羊 Polyclonal DyLight 488

Western Blot of 488 conjugated anti-GFP antibody to detect GFP control proteins. (GFP 抗体 (DyLight 488))

1 image

(22 references)

产品编号 ABIN6720701

Supplier Product No.: 600-141-215

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
165

15

11

9
Aequorea victoria
宿主
62

57

33

13

9

5

1

1
山羊
Compatible Secondaries
克隆类型
109

68
多克隆
标记
79

11

10

10

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to DyLight 488
应用范围
142

73

47

41

26

26

25

22

15

13

13

13

12

11

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Supplier Product No.

600-141-215

Supplier

Rockland

原理

GFP Antibody Dylight™ 488 Conjugated

特异性

Wt, rGFP, eGFP

交叉反应（详细）

Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

纯化方法

GFP Dylight™ 488 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

亚型

IgG

Labeling Ratio

5.7

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应用备注

FLISA_Dilution: >1:10,000
ELISA_Dilution: 1:20,000 - 1:40,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000
Other: User Optimized

说明

Suggested Applications: FC, IF, IHC, IP, Multiplex
Anti-GFP Dylight™ 488 Conjugated Antibody has been tested by dot blot and western blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Conjugated anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Park, Kim, Alabdalla, Mishra, Mchaourab: "Generation and characterization of a zebrafish knockout model of abcb4, a homolog of the human multidrug efflux transporter P-glycoprotein." in: Human genomics, Vol. 17, Issue 1, pp. 84, (2023) (PubMed).

Annunziata, Rasa, Krepelova, Lu, Minetti, Omrani, Nunna, Adam, Käppel, Neri: "Paneth cells drive intestinal stem cell competition and clonality in aging and calorie restriction." in: European journal of cell biology, Vol. 101, Issue 4, pp. 151282, (2022) (PubMed).

Kim, Leng, Park, Sorets, Kim, Shostak, Embalabala, Mlouk, Katdare, Rose, Sturgeon, Neal, Ao, Wang, Sofroniew, Brunger, McMahon, Schrag, Kampmann, Lippmann: "Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin." in: Nature communications, Vol. 13, Issue 1, pp. 6581, (2022) (PubMed).

Paterson, Lämmermann: "Macrophage network dynamics depend on haptokinesis for optimal local surveillance." in: eLife, Vol. 11, (2022) (PubMed).

Kim, Jiang: "Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin." in: STAR protocols, Vol. 2, Issue 2, pp. 100443, (2022) (PubMed).

Bhargava, Williart, Maurin, Davidson, Jouve, Piel, Lahaye, Manel: "Inhibition of HIV infection by structural proteins of the inner nuclear membrane is associated with reduced chromatin dynamics." in: Cell reports, Vol. 36, Issue 13, pp. 109763, (2022) (PubMed).

Lee, Zhao, Wong, Chrysostomou, Crowston, Bui: "The Effect of Aging on Retinal Function and Retinal Ganglion Cell Morphology Following Intraocular Pressure Elevation." in: Frontiers in aging neuroscience, Vol. 14, pp. 859265, (2022) (PubMed).

Wang, Wong, Lee, Bui, Dudczig, Vessey, Fletcher: "Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout." in: Scientific reports, Vol. 11, Issue 1, pp. 4184, (2021) (PubMed).

Freter, Falletta, Omrani, Rasa, Herbert, Annunziata, Minetti, Krepelova, Adam, Käppel, Rüdiger, Wang, Goding, Neri: "Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells." in: Nature communications, Vol. 12, Issue 1, pp. 3318, (2021) (PubMed).

Xu, Brawner, Li, Liu, Kim, Xue, Pang, Kim, Hart, Liu, Jiang: "OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome." in: Cell stem cell, Vol. 24, Issue 6, pp. 908-926.e8, (2020) (PubMed).

Russo, Latour, McGavern: "Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury." in: Nature immunology, Vol. 19, Issue 5, pp. 442-452, (2019) (PubMed).

Fletcher, Greferath, Saha, Anderson, Vessey: "Ganglion Cell Assessment in Rodents with Retinal Degeneration." in: Methods in molecular biology (Clifton, N.J.), Vol. 1753, pp. 261-273, (2019) (PubMed).

Gentili, Lahaye, Nadalin, Nader, Puig Lombardi, Herve, De Silva, Rookhuizen, Zueva, Goudot, Maurin, Bochnakian, Amigorena, Piel, Fachinetti, Londoño-Vallejo, Manel: "The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus." in: Cell reports, Vol. 26, Issue 9, pp. 2377-2393.e13, (2019) (PubMed).

Kolter, Feuerstein, Zeis, Hagemeyer, Paterson, dErrico, Baasch, Amann, Masuda, Lösslein, Gharun, Meyer-Luehmann, Waskow, Franzke, Grün, Lämmermann, Prinz, Henneke: "A Subset of Skin Macrophages Contributes to the Surveillance and Regeneration of Local Nerves." in: Immunity, Vol. 50, Issue 6, pp. 1482-1497.e7, (2019) (PubMed).

Haas, Meiler, Geiger, Vogt, Preuss, Kochs, Pichlmair: "Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity." in: PLoS pathogens, Vol. 14, Issue 4, pp. e1006980, (2018) (PubMed).

Begolly, Olschowka, Love, Williams, OBanion: "Fractionation enhances acute oligodendrocyte progenitor cell radiation sensitivity and leads to long term depletion." in: Glia, Vol. 66, Issue 4, pp. 846-861, (2018) (PubMed).

Bongianino, Denegri, Mazzanti, Lodola, Vollero, Boncompagni, Fasciano, Rizzo, Mangione, Barbaro, Di Fonso, Napolitano, Auricchio, Protasi, Priori: "Allele-Specific Silencing of Mutant mRNA Rescues Ultrastructural and Arrhythmic Phenotype in Mice Carriers of the R4496C Mutation in the Ryanodine Receptor Gene (RYR2)." in: Circulation research, Vol. 121, Issue 5, pp. 525-536, (2017) (PubMed).

Dufour, Gillet, Frankel, Weibel, Emonet: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).

"Correction: Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005149, (2016) (PubMed).

Ho, Vessey, Fletcher: "Immunolocalization of the P2X4 receptor on neurons and glia in the mammalian retina." in: Neuroscience, Vol. 277, pp. 55-71, (2015) (PubMed).

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抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Synonyms: goat anti-GFP Antibody DyLight™ 488 Conjugation, DyLight™ 488 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Background: GFP Dylight™ 488 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt

P42212