GFP 抗体 (DyLight 488)
(5 references)
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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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适用
- Aequorea victoria
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GFP antibody is conjugated to DyLight 488
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应用范围
- Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA, Dot Blot (DB)
- Supplier Product No.
- 600-141-215
- Supplier
- Rockland
- 原理
- GFP Antibody Dylight™ 488 Conjugated
- 特异性
- Wt, rGFP, eGFP
- 交叉反应 （详细）
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Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). - 纯化方法
- GFP Dylight™ 488 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- 亚型
- IgG
- Labeling Ratio
- 5.7
- Top Product
- Discover our top product GFP Primary Antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
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- 应用备注
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FLISA_Dilution: >1:10,000
ELISA_Dilution: 1:20,000 - 1:40,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: 1:10,000
Other: User Optimized
- 说明
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Suggested Applications: FC, IF, IHC, IP, Multiplex
Anti-GFP Dylight™ 488 Conjugated Antibody has been tested by dot blot and western blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store Conjugated anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus." in: Cell reports, Vol. 26, Issue 9, pp. 2377-2393.e13, (2019) (PubMed).
: "Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity." in: PLoS pathogens, Vol. 14, Issue 4, pp. e1006980, (2018) (PubMed).
: "Fractionation enhances acute oligodendrocyte progenitor cell radiation sensitivity and leads to long term depletion." in: Glia, Vol. 66, Issue 4, pp. 846-861, (2018) (PubMed).
: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).
: "New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells." in: Breast cancer research : BCR, Vol. 16, Issue 1, pp. R1, (2015) (PubMed).
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: "The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus." in: Cell reports, Vol. 26, Issue 9, pp. 2377-2393.e13, (2019) (PubMed).
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 背景
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Synonyms: goat anti-GFP Antibody DyLight™ 488 Conjugation, DyLight™ 488 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Background: GFP Dylight™ 488 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- UniProt
- P42212
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