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- 抗原 See all Biotin products
- Biotin
- 适用
- 请咨询
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Biotin antibody is conjugated to DyLight 488
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应用范围
- Western Blotting (WB), Fluorescence Microscopy (FM), FLISA
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
- 免疫原
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Immunogen Type: Other
- 亚型
- IgG
- Labeling Ratio
- 4.0
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anti-Biotin antibody
WB, ELISA, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibodyWB, FACS, IHC, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyELISA 宿主: 山羊 Polyclonal unconjugated Fab fragment
anti-Biotin antibodyFACS, ELISA 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyFACS, ELISA, IP, CyTOF 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
anti-Biotin antibodyIP, Con 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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: "Adnectin-drug conjugates for Glypican-3-specific delivery of a cytotoxic payload to tumors." in: Protein engineering, design & selection : PEDS, Vol. 31, Issue 5, pp. 159-171, (2019) (PubMed).
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: "Adnectin-drug conjugates for Glypican-3-specific delivery of a cytotoxic payload to tumors." in: Protein engineering, design & selection : PEDS, Vol. 31, Issue 5, pp. 159-171, (2019) (PubMed).
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- 抗原
- Biotin
- Abstract
- Biotin 产品
- 物质类
- Chemical
- 背景
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Synonyms: Goat anti Biotin Antibody Dylight™ 488 Conjugated, Goat anti-biotin Antibody Dylight™ 488 Conjugated
Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin DyLight Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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