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His Tag 抗体 (DyLight 649)

Name: His Tag 抗体 (DyLight 649)
SKU: ABIN6720637
Availability: OutOfStock

Rockland 200-343-382 WB, ELISA, DB, FLISA, FM 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649

Western Blot of Mouse anti-6xHIS Tag Antibody. (His Tag 抗体 (DyLight 649))

ELISA

Western Blot of Mouse Anti-6X HIS EPITOPE TAG Antibody 649 Conjugated. (His Tag 抗体 (DyLight 649))

3 images

(2 references)

产品编号 ABIN6720637

Supplier Product No.: 200-343-382

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

请咨询
宿主
96

59

11

9

9

8

2

1
小鼠
Compatible Secondaries
克隆类型
108

74

10
单克隆
标记
93

20

13

13

5

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is conjugated to DyLight 649
应用范围
154

112

39

27

25

19

15

13

13

13

13

10

7

5

4

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Dot Blot (DB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.

200-343-382

Supplier

Rockland

原理

6X His Tag Antibody DyLight™ 649

交叉反应（详细）

This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

纯化方法

This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

免疫原

This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

克隆位点

33D10-D2-G8

亚型

IgG1 kappa

Labeling Ratio

2.5

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应用备注

FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000-1:25,000
Other: FLUORESCENT POLARIZATION: 2 - 20 nM

说明

Suggested Applications: Biochemical Assay, Microarray
Anti-6X HIS tagged Antibody conjugated to DyLight™649 has been tested by ELISA, dot blot, and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Kariolis, Miao, Diep, Nash, Olcina, Jiang, Jones, Kapur, Mathews, Koong, Rankin, Cochran, Giaccia: "Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies." in: The Journal of clinical investigation, Vol. 127, Issue 1, pp. 183-198, (2017) (PubMed).

Kariolis, Miao, Jones, Kapur, Mathews, Giaccia, Cochran: "An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis." in: Nature chemical biology, Vol. 10, Issue 11, pp. 977-83, (2014) (PubMed).
抗原

His Tag

Abstract

His Tag 产品

背景

Synonyms: mouse anti-6X His Tag DyLight™ 649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Background: Anti 6X HIS tagged Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.