RMI2 抗体
-
- 抗原 See all RMI2 抗体
- RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))
- 适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This RMI2 antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for RMI2 detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- KMTDLSDNPI HESMWELEVE DLHRNIP
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
- Rabbit IgG polyclonal antibody for RMI2 detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence of human RMI2(KMTDLSDNPIHESMWELEVEDLHRNIP).
- 亚型
- IgG
- Top Product
- Discover our top product RMI2 Primary Antibody
-
anti-RMI2, RecQ Mediated Genome Instability 2 (RMI2) (AA 1-147) antibody
RMI2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL
- 说明
-
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- 抗原
- RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))
- 别名
- RMI2 (RMI2 产品)
- 别名
- BLAP18 antibody, C16orf75 antibody, C25H16orf75 antibody, C14H16orf75 antibody, A630055G03Rik antibody, Gm537 antibody, Gm929 antibody, RecQ mediated genome instability 2 antibody, RMI2 antibody, Rmi2 antibody
- 背景
-
Synonyms: RecQ-mediated genome instability protein 2, hRMI2, BLM-associated protein of 18 kDa, BLAP18, RMI2, C16orf75
Background: RMI2 is a component of the BLM (RECQL3) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability. This gene is mapped to 16p13.13. RMI1 and RMI2 were present in approximately stoichiometric amounts with other BLM complex components, including topoisomerase-3-alpha (TOP3A), RPA (see RPA1), and BLAP250. RMI2 also associated with RMI1 and TOP3A in a second complex. RMI1 and RMI2 interacted directly, and both were essential for stability of the BLM complex. Depletion of either RMI1 or RMI2 depleted the other protein by 80 to 90 % . Chicken DT40 cells depleted of Rmi2 displayed elevated sister chromatid exchange, but other functions of the BLM complex appeared intact. Mutation analysis revealed that interaction between human RMI2 and BLM was essential for suppression of sister chromatid exchange.
- 基因ID
- 116028
- UniProt
- Q96E14
- 途径
- DNA Damage Repair
-
