Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) 抗体
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- 抗原 See all Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) 抗体
- Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)
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抗原表位
- AA 10-459
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA
- 原理
- Rabbit IgG polyclonal antibody for MT-ND4 detection. Tested with WB, Direct ELISA in Human.
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
- Rabbit IgG polyclonal antibody for MT-ND4 detection. Tested with WB, Direct ELISA in Human.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).
- 亚型
- IgG
- Top Product
- Discover our top product MT-ND4 Primary Antibody
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anti-Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) antibody
MT-ND4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 406-459) antibodyMT-ND4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 350-450) antibodyMT-ND4 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) antibodyMT-ND4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application details: Western blot|0.1-0.5 μg/mL Direct ELISA|0.1-0.5 μg/mL
- 说明
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)
- 别名
- MT-ND4 (MT-ND4 产品)
- 别名
- MTND4 antibody, NADH4 antibody, NADH dehydrogenase, subunit 4 (complex I) antibody, NADH dehydrogenase subunit 4 antibody, NADH dehydrogenasesubunit 4 antibody, ND4 antibody, nad4 antibody, NAD4 antibody
- 背景
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Synonyms: NADH-ubiquinone oxidoreductase chain 4, NADH dehydrogenase subunit 4, MT-ND4, MTND4, NADH4, ND4,
Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial geneMT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.
- 基因ID
- 4538
- UniProt
- P03905
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