NOX5 抗体 (AA 13-197)
-
- 抗原 See all NOX5 抗体
- NOX5 (NADPH Oxidase 5 (NOX5))
-
抗原表位
- AA 13-197
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This NOX5 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- 原理
- Rabbit IgG polyclonal antibody for NOX5 detection. Tested with WB, FCM, Direct ELISA in Human.
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
- Rabbit IgG polyclonal antibody for NOX5 detection. Tested with WB, FCM, Direct ELISA in Human.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human NOX5 recombinant protein (Position: E13-F197).
- 亚型
- IgG
- Top Product
- Discover our top product NOX5 Primary Antibody
-
-
- 应用备注
-
Application details: Western blot|0.1-0.5 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL
- 说明
-
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- 抗原
- NOX5 (NADPH Oxidase 5 (NOX5))
- 别名
- NOX5 (NOX5 产品)
- 别名
- NADPH oxidase 5 antibody, NOX5 antibody
- 背景
-
Synonyms: NADPH oxidase 5, NOX5
Background: NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.
- 途径
- Proton Transport
-