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DVL1 抗体 (AA 21-100)

Name: DVL1 抗体 (AA 21-100)
SKU: ABIN670671
Availability: OutOfStock
Rating: 5 (1 reviews)

DVL1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671) (DVL1 抗体 (AA 21-100))

IHC

Images provided the Independent Validation Program (badge number 029657)Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-DVL1 Polyclonal Antibody (ABIN670671) at 1:250 overnight at room temperature followed by conjugation to secondary antibody. (DVL1 抗体 (AA 21-100))

IHC

Lane 1: rat brain lysates Lane 2: rat lung lysates probed with Anti DVL1/Dishevelled Polyclonal Antibody, Unconjugated (ABIN670671) at 1:200 in 4 °C. (DVL1 抗体 (AA 21-100))

SDS

6 images

(1 reference)

(1 validation)

产品编号 ABIN670671

发货至: 中国

Datasheet as PDF

抗原 See all DVL1 抗体

DVL1 (Dishevelled Segment Polarity Protein 1 (DVL1))
抗原表位
15

7

7

7

6

3

2

2

1

1

1

1

1

1

1

1
AA 21-100
适用
48

29

25

3

3

3

2

2

2

1

1

1
人, 小鼠, 大鼠
宿主
46

2

2
兔
Compatible Secondaries
克隆类型
50
多克隆
标记
19

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This DVL1 antibody is un-conjugated
应用范围
43

18

18

13

13

12

12

7

4

2

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human DVL1

亚型

IgG
anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 442-470) antibody
DVL1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB31716 unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 150-285) antibody
DVL1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 20-32) antibody
Verified DVL1 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 510-640) antibody
DVL1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 1-110) antibody
DVL1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 1-444) antibody
DVL1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (Internal Region) antibody
Verified DVL1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 1-444) antibody
DVL1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 70-120) antibody
DVL1 适用: 人, 小鼠, 大鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 569-618) antibody
DVL1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029657 (Immunohistochemistry)

by

Immunohistochemistry Core, NYU Langone

No.

#029657

日期

2014.04.03

抗原

Lot Number

140106

Method validated

Immunohistochemistry

Positive Control

Human kidney tubules

Negative Control

Human colon stromal tissue

Notes

While faint, signal is detectable in positive control tissue and not in negative control tissue. Note that staining is expected in colon epithelial cells, and is not expected in kidney glomeruli.

Validation Images

Figure 1: Human kidney tissue stained with anti-DVL1 (brown) and counterstained with hematoxylin.

Figure 2: Human colon tissue stained with anti-DVL1 (brown) and counterstained with hematoxylin.

Figure 3: Human kidney tissue stained with isotype control antibody (brown) and counterstained with hematoxylin.

Figure 4: Human kidney tissue stained with secondary only (brown) and counterstained with hematoxylin.
Full Methods
Primary Antibody

Antigen: Dishevelled, Dsh Homolog 1 (Drosophila) (DVL1)
Catalog number: ABIN670671
Lot number: 140106

Secondary Antibody

Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
Lot number: D07640BA

Full Protocol

Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
1. Slides were preheated in convection oven at 60°C for 30 min
2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
5. Slides rinsed with *reaction buffer* x3
6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
7. Slides rinsed with *reaction buffer*
8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
9. Slides rinsed with *reaction buffer* x3
10. *iView Biotinylated IgG* applied and incubated for 8 min
11. Slides rinsed with *reaction buffer*
14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
15. Slides rinsed with *reaction buffer*
16. *iView DAB/H2O2* applied and incubated for 8 min
17. Slides rinsed with *reaction buffer*
18. *iView Copper* applied and incubated for 4 min
19. Slides rinsed with *reaction buffer*
20. Slides washed in Dawn Detergent/tap water
21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software

Experimental Notes

Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
Step 4: Italicized reagents and incubation time are fixed instrument parameters.
Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Hua, Xu, He, Jiang, Ye, Pan: "Wnt4/?-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8421-31, (2015) (PubMed).
抗原

DVL1 (Dishevelled Segment Polarity Protein 1 (DVL1))

别名

DVL1 (DVL1 产品)

别名

DVL antibody, DVL1L1 antibody, DVL1P1 antibody, Dvl antibody, mKIAA4029 antibody, dvl-1 antibody, DSH antibody, DVL-1 antibody, dvl1 antibody, dvl2l antibody, Xdsh antibody, dsh1 antibody, dishevelled segment polarity protein 1 antibody, dishevelled, dsh homolog 1 (Drosophila) antibody, microRNA 6808 antibody, dishevelled segment polarity protein 1b antibody, dishevelled segment polarity protein 1 L homeolog antibody, DVL1 antibody, Dvl1 antibody, MIR6808 antibody, dvl1b antibody, dvl1.L antibody

背景

Synonyms: DVL, DVL1L1, DVL1P1, Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1
Background: Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).

基因ID

1855

UniProt

O14640

途径

WNT signaling, Synaptic Membrane, Skeletal Muscle Fiber Development