PALB2 抗体 (AA 1101-1186)
(1 validation)-
- 抗原 See all PALB2 抗体
- PALB2 (Partner and Localizer of BRCA2 (PALB2))
-
抗原表位
- AA 1101-1186
-
适用
- 人, Pig
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This PALB2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 人, Pig
- 预测反应
- Mouse,Rat,Cow
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human PALB2
- 亚型
- IgG
-
anti-Partner and Localizer of BRCA2 (PALB2) (AA 677-726) antibody
PALB2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Partner and Localizer of BRCA2 (PALB2) (AA 675-725) antibodyPALB2 适用: 人 WB, IHC, IP, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Partner and Localizer of BRCA2 (PALB2) (AA 99-198) antibodyPALB2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Partner and Localizer of BRCA2 (PALB2) (AA 546-574) antibodyPALB2 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19074 unconjugated
anti-Partner and Localizer of BRCA2 (PALB2) (C-Term) antibodyPALB2 适用: 人, 小鼠, 大鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated
anti-Partner and Localizer of BRCA2 (PALB2) (AA 553-582), (Middle Region) antibodyPALB2 适用: 人 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
-
- by
- Alamo Laboratories Inc
- No.
- #029749
- 日期
- 2014.07.01
- 抗原
- Lot Number
- 120510
- Method validated
- Western Blotting
- Positive Control
- A549 cell extract
- Negative Control
- c6/36 mosquito cell extract (non-reactive species)
- Notes
- A single strong band was observed in the positive control at the correct molecular weight. Several faint bands appeared in the negative control, but they were much less intense than the positive control band.
- Primary Antibody
- Antigen: Partner and Localizer of BRCA2 (PALB2) (1:500 dilution)
- Catalog number: ABIN670536
- Lot number: 120510
- Secondary Antibody
- Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
- Lot number: L170-6515
- Full Protocol
- 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
- 2. 35 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
- 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
- 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
- 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
- 6. The membrane was rinsed with TBST once.
- 7. The membrane was immersed with the protein side up in the primary antibody solution (anti-PALB2; 1:500) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
- 8. The membrane was rinsed in TBST thrice for 5 min each.
- 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
- 10. The membrane was rinsed thrice TBST thrice for 5 min each.
- 11. The membrane was rinsed in TBS twice for 30 s each.
- 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
- 13. The membrane was rinsed three times TBST.
- 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
- 15. The membrane was washed in TBST 2 times for 10 min each.
- 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
- Experimental Notes
- - No challenges noted.
生效 #029749 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1. Western blot of lysates from A549 cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-PALB2 (upper panel) or with anti-Actin for loading control (lower panel).]()
Figure 1. Western blot of lysates from A549 cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-PALB2 (upper panel) or with anti-Actin for loading control (lower panel).
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
-
- 抗原
- PALB2 (Partner and Localizer of BRCA2 (PALB2))
- 别名
- PALB2 (PALB2 产品)
- 别名
- PALB2 antibody, Fancn antibody, si:ch211-14k19.9 antibody, FANCN antibody, PNCA3 antibody, 4732427B05 antibody, BC066140 antibody, RGD1304759 antibody, partner and localizer of BRCA2 antibody, partner and localizer of BRCA2 L homeolog antibody, PALB2 antibody, palb2 antibody, palb2.L antibody, Palb2 antibody
- 背景
-
Synonyms: FANCN, PNCA3, Partner and localizer of BRCA2, PALB2
Background: Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity.
- 基因ID
- 79728
- UniProt
- Q86YC2
- 途径
- DNA Damage Repair
-
