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MERTK 抗体 (AA 501-600)

Name: MERTK 抗体 (AA 501-600)
SKU: ABIN669966
Availability: OutOfStock
Rating: 5 (1 reviews)

MERTK 适用: 小鼠 ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) (AA 501-600) antibody (ABIN669966) (MERTK 抗体 (AA 501-600))

IHC

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti C-Mer/MERTK Polyclonal Antibody, Unconjugated (ABIN669966) at 1:200 followed by conjugation to the secondary antibody and DAB staining (MERTK 抗体 (AA 501-600))

IHC

2 images

(1 validation)

产品编号 ABIN669966

发货至: 中国

Datasheet as PDF

抗原 See all MERTK 抗体

MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))
抗原表位
13

11

8

8

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 501-600
适用
73

36

28

4

2

2

1

1

1

1

1
小鼠
宿主
66

10

2

1
兔
Compatible Secondaries
克隆类型
59

20
多克隆
标记
45

9

5

4

2

2

2

2

2

2

2

2
This MERTK antibody is un-conjugated
应用范围
51

44

33

14

12

9

8

8

7

3

1

1

1

1
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

小鼠

预测反应

Human,Rat

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human of human MERTK

亚型

IgG

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anti-C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) (AA 840-999) antibody
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anti-C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) (AA 21-120) antibody
MERTK 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D2 unconjugated

anti-C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) antibody
MERTK 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB17701 unconjugated

anti-C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) (AA 946-980), (C-Term) antibody
MERTK 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB50416 unconjugated

anti-C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) (Extracellular Domain) antibody
MERTK 适用: 人 WB 宿主: 小鼠 Monoclonal A311F9G3 unconjugated

anti-C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) antibody
MERTK 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7E5G1 unconjugated

应用备注

ELISA 1:500-1000
IHC-P 1:200-400

限制

仅限研究用
生效 #104528 (Immunohistochemistry)

by

Palczewski Lab, Center For Translational Vision Research, UC Irvine

No.

#104528

日期

2023.12.13

抗原

MERTK

Lot Number

AI08084045

Method validated

Immunohistochemistry

Positive Control

Retina cryosection from B6 Albino (B6(Cg)-Tyrc-2J/J) animal

Negative Control

Retina cryosection from Mertk-ko Albino animal (B6(Cg)-Tyrc-2J/J background).

Notes

Passed. Presence of specific signal in the RPE cell layer was considered as indication of specific immunoreactivity.

Validation Images

Retinal sections from the wild-type (B6 albino) and Mertk knock-out (albino) mice immunostained with anti-MERTK antibody ABIN669966. DAPI staining shows localization of the inner (INL) and outer (ONL) nuclear layer of the mouse retina. PNA co-staining was used to visualize cone inner and outer segments (IS/OS) in the retina. Presence of specific signal in the RPE cell layer confirms specific immunoreactivity.
Full Methods
Primary Antibody

ABIN669966

Secondary Antibody

anti-rabbit secondary antibody Alexa Fluor 647 (Thermo Fisher Scientific A32795)

Full Protocol

Collect eyes from mice and fix with paraformaldehyde 4% (Electron Microscopy Sciences, 15710) in 1x PBS for 30 min at RT
Cryoprotection with sucrose series:
Wash in 10% sucrose in 1x PBS
Immerse in 10% sucrose in 1x PBS for 30 min at RT
Wash in 20% sucrose in 1x PBS
Immerse in 20% sucrose in 1x PBS for 30 min RT
Wash in 30% sucrose in 1x PBS
30% sucrose ON at 4°C
Embed eyes in OCT compound (Tissue-Tek O.C.T. Compound, 4583)
Cut retinal sections at a thickness of 12 μm on a cryostat
Air dry sections for 15 min at RT, store at -80°C until use
Bring sections to RT and rehydrate in 1x PBS for 1 h
Incubate sections in blocking buffer (1x PBS, 3% BSA (Sigma-Aldrich, A7030), 3% Donkey serum (Sigma-Aldrich, S30-100ML), 0.1% Triton X-100 (Sigma-Aldrich, X100-500ML)) for 1 h at RT
Incubate sections with primary rabbit anti-MERTK antibody diluted 1:100 in blocking buffer ON at RT. Include MERTK knock-out or no primary antibody negative controls
Rinse sections 3 times with 1x PBS, 0.1% Triton X100. Keep negative controls in a separate container
Incubate sections with AF647-conjugated donkey anti-rabbit antibody (Thermo Fisher, A32795, 1:500) and Peanut Agglutinin (PNA) with Rhodamine (Vector Laboratories, RL-1072-5, 1:250) diluted in blocking buffer for 2 h at RT
Rinse sections once with 1x PBS, 0.1% Triton X-100 for 5 min at RT
Mount sections in VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI (Vector Laboratories, H-1500)
Acquire images with a fluorescence microscope and appropriate filter settings. For the validation purposes Keyence BZ-X800E fluorescence microscope was used with following filters: BZ-X DAPI for DAPI, BZ-X TRITC for Rhodamine, BZ-X Cy5 for AF647. Images were taken at 40x magnification

Experimental Notes

Experiment involved validation of the specificity of anti-MERTK antibody. MERTK protein is important for eye function and highly expressed specifically in the RPE. Validation is based on comparison of staining performed on wild-type and MERTK-ko cryosections of the mouse retina.
To aid orientation in the cell layers PNA counterstain was included to visualize cone inner and outer segments (IS/OS) that are located adjacent to the RPE.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

别名

MERTK (MERTK 产品)

别名

MER antibody, RP38 antibody, c-mer antibody, Eyk antibody, Mer antibody, Nyk antibody, nmf12 antibody, rdy antibody, C-EYK antibody, MERTK antibody, DKFZp469D2215 antibody, si:ch1073-268l12.2 antibody, MER proto-oncogene, tyrosine kinase antibody, c-mer proto-oncogene tyrosine kinase antibody, c-mer proto-oncogene tyrosine kinase a antibody, MERTK antibody, Mertk antibody, mertka antibody

背景

Synonyms: MER, RP38, c-Eyk, c-mer, Tyro12, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, MERTK
Background: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

基因ID

10461

UniProt

Q12866

途径

RTK signaling