YDJ1 抗体
-
- 抗原 See all YDJ1 products
- YDJ1 (HSP40 / YDJ1 (YDJ1))
- 适用
- Saccharomyces cerevisiae
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This YDJ1 antibody is un-conjugated
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB), ELISA
- 纯化方法
- Anti-Hsp40, YDJ1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Hsp40 from yeast based on 100% homology with the immunizing sequence. Cross-reactivity with Hsp40 from other sources has not been determined. Heat Shock research.
- 免疫原
-
Immunogen: Hsp40 (YDJ1) Antibody was produced in mice by repeated immunizations raised against full length protein yeast Hsp40 (YDJ1).
Immunogen Type: Recombinant Protein
- 克隆位点
- 1G10-H8
- 亚型
- IgG1
-
-
- 应用备注
-
Application Note: Anti-Hsp40 Antibody is recommended for use in WB, IP, and ELISA. Expect a band approximately ~40 kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:200
Western Blot Dilution: 1:2000
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 50 % (v/v) Glycerol0.09 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
- 抗原
- YDJ1 (HSP40 / YDJ1 (YDJ1))
- 别名
- Hsp40 (YDJ1) (YDJ1 产品)
- 别名
- HSP40 antibody, MAS5 antibody, type I HSP40 co-chaperone YDJ1 antibody, YDJ1 antibody
- 背景
-
Synonyms: Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C
Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding, type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1.
Gene Name: YDJ1
- 基因ID
- 855661
- NCBI登录号
- NP_014335
- UniProt
- P25491
-
