CD68 抗体 (Internal Region)
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- 抗原 See all CD68 抗体
- CD68 (CD68 Molecule (CD68))
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抗原表位
- Internal Region
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD68 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-r10
- Supplier
- Rockland
- 原理
- Cd68 Antibody
- 交叉反应 (详细)
- Anti-CD68 antibody is directed against mouse CD68 protein supplied in the buffer above with 4mg trehalose.
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 免疫原
- CD68 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse CD68.
- 亚型
- IgG
- Top Product
- Discover our top product CD68 Primary Antibody
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- 应用备注
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Immunohistochemistry_Dilution: 0.5-1.0 μg/mL
Flow_Cytometry_Dilution: 1-3 μg/1 million cells
IF_Microscopy_Dilution: 5 μg/mL
Western_Blot_Dilution: 0.1-0.5 μg/mL
- 说明
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Suggested Applications: Other
Anti-CD68 is tested for IHC-P, IHC-Fr, IF, Flow Cytometry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~34.8 kDa corresponding to the appropriate cell lysate or extract. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 200μL
- 缓冲液
- Optional[Buffer]: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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Polarized microtubule remodeling transforms the morphology of reactive microglia and drives cytokine release." in: Nature communications, Vol. 14, Issue 1, pp. 6322, (2023) (PubMed).
: "Targeting Oxidative Stress, Cytokines and Serotonin Interactions Via Indoleamine 2, 3 Dioxygenase by Coenzyme Q10: Role in Suppressing Depressive Like Behavior in Rats." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 12, Issue 2, pp. 277-291, (2018) (PubMed).
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Polarized microtubule remodeling transforms the morphology of reactive microglia and drives cytokine release." in: Nature communications, Vol. 14, Issue 1, pp. 6322, (2023) (PubMed).
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- 抗原
- CD68 (CD68 Molecule (CD68))
- 别名
- CD68 (CD68 产品)
- 背景
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Synonyms: Macrosialin, Gp110, CD68
Background: CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease. This antibody is suitable for researchers interested in cancer research.
Gene Name: CD68
- UniProt
- P31996
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