SIX3 抗体 (Internal Region)
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- 抗原 See all SIX3 抗体
- SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
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抗原表位
- Internal Region
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SIX3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- 纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with mouse Six3. Cross-reactivity with Six3 from other sources has not been determined.
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product SIX3 Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1:100-1:250
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:10,000-1:15,000
Western Blot Dilution: 1:500 - 1:2,000
IF Microscopy Dilution: 1:200 - 1:1,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 4968658, (2018) (PubMed).
: "Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation." in: Cell reports, Vol. 25, Issue 9, pp. 2510-2523.e4, (2018) (PubMed).
: "SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression." in: Development (Cambridge, England), Vol. 145, Issue 14, (2018) (PubMed).
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Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 4968658, (2018) (PubMed).
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- 抗原
- SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
- 别名
- Six3 (SIX3 产品)
- 别名
- HPE2 antibody, SIX3 antibody, cb347 antibody, six3.2 antibody, six6 antibody, E130112M24Rik antibody, Six3a antibody, Six3alpha antibody, Six3b antibody, Six3beta antibody, six3 antibody, six3.1 antibody, wu:fc10a08 antibody, SIX homeobox 3 antibody, SIX homeobox 3b antibody, sine oculis-related homeobox 3 antibody, SIX homeobox 3a antibody, sine oculis homeobox homolog 3 antibody, SIX3 antibody, six3b antibody, Six3 antibody, six3a antibody, six3 antibody
- 背景
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Synonyms: rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3
Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain. Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product. Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.
Gene Name: Six3
- 基因ID
- 20473, 59939908
- UniProt
- Q62233
- 途径
- Protein targeting to Nucleus
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