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SIX3 抗体 (Internal Region)

Rockland 600-401-a26 SIX3 适用: 小鼠 WB, ELISA, IHC, FM 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN6657912
Supplier Product No.: 600-401-a26
发货至: 中国
  • 抗原 See all SIX3 抗体
    SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
    抗原表位
    • 15
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 36
    • 11
    • 9
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    小鼠
    宿主
    • 35
    • 3
    • 1
    • 1
    克隆类型
    • 38
    • 2
    多克隆
    标记
    • 21
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIX3 antibody is un-conjugated
    应用范围
    • 39
    • 16
    • 13
    • 13
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-a26
    Supplier
    Rockland
    原理
    Six3 Antibody
    交叉反应 (详细)
    This antibody reacts with mouse Six3.
    纯化方法
    This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    过滤
    Sterile filtered
    免疫原
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
    亚型
    IgG
    Top Product
    Discover our top product SIX3 Primary Antibody
  • 应用备注

    ELISA_Dilution: 1:10,000-1:15,000

    Immunohistochemistry_Dilution: 1:100-1:250

    IF_Microscopy_Dilution: 1:200 - 1:1,000

    Western_Blot_Dilution: 1:500 - 1:2,000

    Other: User Optimized

    说明

    Suggested Applications: IF, Multiplex
    This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Li, Xie, He, Zhou, Gao, Liu, Pan, Ge, Peng, Zhong: "Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 4968658, (2018) (PubMed).

    Diacou, Zhao, Zheng, Cvekl, Liu: "Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation." in: Cell reports, Vol. 25, Issue 9, pp. 2510-2523.e4, (2018) (PubMed).

    Xu, Liang, Song, Zhang, Lindtner, Li, Wen, Liu, Guo, Qi, Wang, Wang, Li, You, Wang, Chen, Feng, Rubenstein, Yang: "SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression." in: Development (Cambridge, England), Vol. 145, Issue 14, (2018) (PubMed).

  • 抗原
    SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
    别名
    Six3 (SIX3 产品)
    背景

    Synonyms: rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3

    Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

    Gene Name: Six3

    基因ID
    20473, 59939908
    UniProt
    Q62233
    途径
    Protein targeting to Nucleus
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