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NK-1.1/CD161c 抗体 (APC)

Rockland 200-326-n93 适用: 小鼠 FACS, IP, IHC, FM 宿主: 小鼠 Monoclonal PK136 APC
Rockland
产品编号 ABIN6657869
Supplier Product No.: 200-326-n93
发货至: 中国
  • 抗原 See all NK-1.1/CD161c products
    NK-1.1/CD161c (NK-1.1)
    适用
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    小鼠
    宿主
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    小鼠
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    单克隆
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    This NK-1.1/CD161c antibody is conjugated to APC
    应用范围
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    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    Supplier Product No.
    200-326-n93
    Supplier
    Rockland
    原理
    NK1.1 Allophycocyanin Antibody
    纯化方法
    Allophycocyanin conjugated NK1.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse NK1.
    过滤
    Sterile filtered
    免疫原
    Anti-NK1.1 Antibody (Monoclonal) was produced by repeated immunizations with NK1.1 antigen.
    克隆位点
    PK136
    亚型
    IgG2a kappa
    Labeling Ratio
    1-2
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    IF_Microscopy_Dilution: User Optimized

    说明

    Anti-NK1.1 is tested in Flow Cytometry useful for Immunohistochemistry, Immunoprecipitation, Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.1 % Gelatin

    Preservative: 0.09 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
    有效期
    6 months
  • 抗原
    NK-1.1/CD161c (NK-1.1)
    别名
    NK1.1 (NK-1.1/CD161c 产品)
    背景

    Synonyms: Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c

    Background: The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

    Gene Name: Klrb1c

    基因ID
    17059
    NCBI登录号
    NP_001153376
    UniProt
    P27814
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