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DAB1 抗体 (AA 400-555)

Name: DAB1 抗体 (AA 400-555)
SKU: ABIN6657863
Availability: OutOfStock

DAB1 适用: 小鼠 WB, ELISA, IHC, IP, FM 宿主: 兔 Polyclonal unconjugated

WB - Dab1 Antibody Immunochemical's anti-Dab1 is shown to detect Dab1 present in wt mouse brain extracts (lane 1). (DAB1 抗体 (AA 400-555))

IHC - Dab1 Antibody Immunohistochemical staining after cryofixation and sectioning of mouse brain tissue using (A) a 1:5,000 dilution of anti-Dab-1 and (B) 1:5,000 dilution of pre-immune serum followed by processing with HRP Goat anti-Rabbit IgG [H&L] and chromogenic substrate. (DAB1 抗体 (AA 400-555))

WB - Dab1 Antibody Analysis of GFP-Dab1 and endogenous Dab1 levels in transfected retinal cells and retinal tissue. (DAB1 抗体 (AA 400-555))

3 images

(6 references)

产品编号 ABIN6657863

发货至: 中国

Datasheet as PDF

抗原 See all DAB1 抗体

DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))
抗原表位
15

15

15

7

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 400-555
适用
55

53

52

4

3

3

3

3

3

2

2

1
小鼠
宿主
86

3

3
兔
Compatible Secondaries
克隆类型
91

1
多克隆
标记
43

5

5

4

3

3

3

3

3

3

3

3

3

3

3

1

1
This DAB1 antibody is un-conjugated
应用范围
70

41

41

34

22

18

11

8

6

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

原理

Dab1 Antibody

交叉反应（详细）

This antibody is directed against Dab1 from mouse.

纯化方法

Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.

过滤

Sterile filtered

免疫原

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.

亚型

IgG

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anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr232) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 400-555) antibody
DAB1 适用: 小鼠 WB, ELISA, IHC, IP, FM 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr232) antibody
DAB1 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (Ser296) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 1-185) antibody
DAB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr198) antibody
DAB1 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (Ser295) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 1-553) antibody
DAB1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 187-236) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (C-Term) antibody
DAB1 适用: 人, 小鼠, 大鼠, Pig, 猴, 马, 兔, Cow, 犬, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Immunoprecipitation_Dilution: 1 μL per 500 μg
ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:5,000
IF_Microscopy_Dilution: User Optimized
Western_Blot_Dilution: 1:5,000
Other: User Optimized

说明

Suggested Applications: Biochemical Assay, IF, Multiplex
Anti-Dab1 antibody has been tested by western blot and immunohistochemistry and is suitable for the detection of Dab1 by immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate. For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature. For immunoprecipitation use 1 μl of antiserum per 500 μg of brain lysate. Perform immunoprecipitation at 4°C for 2 h. For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 μg/ml aprotinin and 10 μg/ml leupeptin.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Meseke, Neumüller, Brunne, Li, Anstötz, Pohlkamp, Rogalla, Herz, Rune, Bender: "Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin." in: eNeuro, Vol. 5, Issue 5, (2019) (PubMed).

Fairchild, Hino, Han, Miltner, Peinado Allina, Brown, Burns, La Torre, Simó: "RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion." in: Development (Cambridge, England), Vol. 145, Issue 3, (2018) (PubMed).

Katyal, Gao, Monckton, Glubrecht, Godbout: "Hierarchical disabled-1 tyrosine phosphorylation in Src family kinase activation and neurite formation." in: Journal of molecular biology, Vol. 368, Issue 2, pp. 349-64, (2007) (PubMed).

Bramblett, Pennesi, Wu, Tsai: "The transcription factor Bhlhb4 is required for rod bipolar cell maturation." in: Neuron, Vol. 43, Issue 6, pp. 779-93, (2004) (PubMed).

Keshvara, Magdaleno, Benhayon, Curran: "Cyclin-dependent kinase 5 phosphorylates disabled 1 independently of Reelin signaling." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 12, pp. 4869-77, (2002) (PubMed).

Sheldon, Rice, DArcangelo, Yoneshima, Nakajima, Mikoshiba, Howell, Cooper, Goldowitz, Curran: "Scrambler and yotari disrupt the disabled gene and produce a reeler-like phenotype in mice." in: Nature, Vol. 389, Issue 6652, pp. 730-3, (1997) (PubMed).
抗原

DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

别名

Dab1 (DAB1 产品)

别名

AI956902 antibody, C630028C02Rik antibody, scm antibody, scr antibody, scrambler antibody, yot antibody, disabled-1 antibody, LOC100232823 antibody, si:dkey-242m13.1 antibody, DAB1 antibody, zgc:136547 antibody, DAB1, reelin adaptor protein antibody, disabled 1 antibody, disabled homolog 1 antibody, Dab, reelin signal transducer, homolog 1a (Drosophila) antibody, Dab, reelin signal transducer, homolog 1b (Drosophila) antibody, DAB1 antibody, Dab1 antibody, LOC100232823 antibody, dab1a antibody, dab1b antibody

背景

Synonyms: rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody
Background: Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'. Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes. Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development. Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain. Cloning of human Dab1 and sequence determinations suggest a 96 % identity to the mouse sequence. Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins. Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2. In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.
Gene Name: DAB1

基因ID

13131

NCBI登录号

NP_034144

UniProt

P97318