CD3 epsilon 抗体 (FITC)
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- 抗原 See all CD3 epsilon (CD3E) 抗体
- CD3 epsilon (CD3E)
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适用
- 小鼠
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宿主
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仓鼠
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克隆类型
- 单克隆
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标记
- This CD3 epsilon antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- Supplier Product No.
- 200-b02-m69
- Supplier
- Rockland
- 原理
- CD3e Fluorescein Antibody
- 交叉反应 (详细)
- Reactivity is observed against mouse CD3.
- 纯化方法
- Fluorescein conjugated CD3 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD3.
- 过滤
- Sterile filtered
- 免疫原
- Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with H-2Kb specific cytotoxic T lymphocyte clone BM10-37.
- 克隆位点
- 145-2C11
- 亚型
- IgG
- Labeling Ratio
- 2-8
- Top Product
- Discover our top product CD3E Primary Antibody
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- 应用备注
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Flow_Cytometry_Dilution: 0.1-0.5 μg/10^6 cells
Other: Sufficient to run approximately 500 tests
- 说明
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Anti-CD3 is tested for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
- 有效期
- 6 months
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3e (CD3E 产品)
- 背景
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Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Background: CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.
Gene Name: Cd3e
- 基因ID
- 12501
- NCBI登录号
- NP_031674
- UniProt
- P22646
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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