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MCL-1 抗体 (Internal Region)

Name: MCL-1 抗体 (Internal Region)
SKU: ABIN6657787
Availability: OutOfStock

Rockland 600-401-394s MCL1 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Characterization of clonal lymphoid lines mutant for combinations of pro-apoptotic BCL2 family proteins. (MCL-1 抗体 (Internal Region))

ELISA

Rabbit anti-Mcl-1 WB Western Blot of Rabbit anti-Mcl-1 antibody. (MCL-1 抗体 (Internal Region))

Anti-Mcl-1 Antibody - Western Blot. (MCL-1 抗体 (Internal Region))

4 images

(86 references)

产品编号 ABIN6657787

Supplier Product No.: 600-401-394s

发货至: 中国

Datasheet as PDF

抗原 See all MCL-1 (MCL1) 抗体

MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
抗原表位
27

16

16

11

8

6

5

5

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
139

64

62

5

3

3

2

1

1

1
小鼠
宿主
131

11

1

1
兔
Compatible Secondaries
克隆类型
124

22
多克隆
标记
69

9

9

7

5

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This MCL-1 antibody is un-conjugated
应用范围
113

55

46

43

27

26

22

21

11

8

7

5

4
Western Blotting (WB), ELISA

Supplier Product No.

600-401-394s

Supplier

Rockland

原理

Mcl-1 Antibody

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

纯化方法

Anti-Mcl-1 affinity purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.

过滤

Sterile filtered

免疫原

This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

亚型

IgG

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MCL1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (Center) antibody
MCL1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibody
MCL1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibody
MCL1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, IP, ICC 宿主: 兔 Monoclonal unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 19-244) antibody
MCL1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 120-220) antibody
MCL1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibody
MCL1 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 8C6D4B1 unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 191-226) antibody
MCL1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 6-328) antibody
MCL1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA_Dilution: 1:10,000 - 1:50,000
Western_Blot_Dilution: 1:10,000
Other: User Optimized

说明

Suggested Applications: FC, IF, IHC, IP
Anti-Mcl-1 Antibody has been tested by ELISA and western blot and is suitable for immunoprecipitation. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. Cross reactivity with Mcl-1 from other sources is unknown. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

有效期

12 months
Karbon, Schuler, Braun, Eichin, Haschka, Drach, Sotillo, Geley, Spierings, Tijhuis, Foijer, Villunger: "Chronic spindle assembly checkpoint activation causes myelosuppression and gastrointestinal atrophy." in: EMBO reports, Vol. 25, Issue 6, pp. 2743-2772, (2024) (PubMed).

Lindenboim, Zohar, Gundersen, Worman, Stein: "LINC complex protein nesprin-2 has pro-apoptotic activity via Bcl-2 family proteins." in: Cell death discovery, Vol. 10, Issue 1, pp. 29, (2024) (PubMed).

Prucsi, Zimny, Płonczyńska, Zubrzycka, Potempa, Sochalska: "Porphyromonas gingivalis Peptidyl Arginine Deiminase (PPAD) in the Context of the Feed-Forward Loop of Inflammation in Periodontitis." in: International journal of molecular sciences, Vol. 24, Issue 16, (2023) (PubMed).

Quarato, Mari, Barrows, Yang, Ruehl, Chen, Guy, Low, Chen, Green: "Mitophagy restricts BAX/BAK-independent, Parkin-mediated apoptosis." in: Science advances, Vol. 9, Issue 21, pp. eadg8156, (2023) (PubMed).

Huang, Chin, Reljic, Djajawi, Tan, Gong, Stroud, Huang, van Delft, Dewson: "Mitochondrial E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins." in: Cell death and differentiation, Vol. 30, Issue 3, pp. 632-646, (2023) (PubMed).

Pileggi, Parmar, Elkhatib, Stewart, Alecu, Côté, Bennett, Sandhu, Cuperlovic-Culf, Harper: "The SARS-CoV-2 spike glycoprotein interacts with MAO-B and impairs mitochondrial energetics." in: Current research in neurobiology, Vol. 5, pp. 100112, (2023) (PubMed).

Prew, Adhikary, Choi, Portero, Paulo, Gowda, Budhraja, Opferman, Gygi, Danial, Walensky: "MCL-1 is a master regulator of cancer dependency on fatty acid oxidation." in: Cell reports, Vol. 41, Issue 1, pp. 111445, (2022) (PubMed).

Quarato, Llambi, Guy, Min, Actis, Sun, Narina, Pruett-Miller, Peng, Rankovic, Green: "Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak." in: Cell death and differentiation, Vol. 29, Issue 7, pp. 1318-1334, (2022) (PubMed).

Moyzis, Lally, Liang, Najor, Gustafsson: "Mcl-1 Differentially Regulates Autophagy in Response to Changes in Energy Status and Mitochondrial Damage." in: Cells, Vol. 11, Issue 9, (2022) (PubMed).

Buitrago-Molina, Marhenke, Becker, Geffers, Itzel, Teufel, Jaeschke, Lechel, Unger, Markovic, Sharma, Marquardt, Saborowski, Saborowski, Vogel: "p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model." in: Cellular and molecular gastroenterology and hepatology, Vol. 11, Issue 5, pp. 1387-1404, (2022) (PubMed).

Smith, Budhraja, Lynch, Roberts, Panetta, Connelly, Turnis, Pruett-Miller, Schuetz, Mullighan, Opferman: "The Heme-Regulated Inhibitor Pathway Modulates Susceptibility of Poor Prognosis B-Lineage Acute Leukemia to BH3-Mimetics." in: Molecular cancer research : MCR, Vol. 19, Issue 4, pp. 636-650, (2022) (PubMed).

Teh, Robbins, Henstridge, Dewson, Diepstraten, Kelly, Febbraio, Gabriel, O'Reilly, Strasser, Gray: "MCL-1 is essential for survival but dispensable for metabolic fitness of FOXP3+ regulatory T cells." in: Cell death and differentiation, Vol. 27, Issue 12, pp. 3374-3385, (2021) (PubMed).

Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

Djajawi, Liu, Gong, Huang, Luo, Xu, Okamoto, Call, Huang, van Delft: "MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1:NOXA complex." in: Cell death and differentiation, Vol. 27, Issue 8, pp. 2484-2499, (2021) (PubMed).

Moyzis, Lally, Liang, Leon, Najor, Orogo, Gustafsson: "Mcl-1-mediated mitochondrial fission protects against stress but impairs cardiac adaptation to exercise." in: Journal of molecular and cellular cardiology, Vol. 146, pp. 109-120, (2021) (PubMed).

Mizutani, Hikita, Saito, Myojin, Sato, Urabe, Kurahashi, Shiode, Sakane, Murai, Nozaki, Kodama, Sakamori, Yoshida, Tatsumi, Takehara: "Gab1 in livers with persistent hepatocyte apoptosis has an antiapoptotic effect and reduces chronic liver injury, fibrosis, and tumorigenesis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 320, Issue 6, pp. G958-G968, (2021) (PubMed).

Sumiyoshi, Kotani, Ikuta, Suzue, Ozawa, Katakai, Yamada, Abe, Bando, Koyasu, Kanaho, Watanabe, Matsuda: "Arf1 and Arf6 Synergistically Maintain Survival of T Cells during Activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 206, Issue 2, pp. 366-375, (2021) (PubMed).

Hirsova, Bohm, Dohnalkova, Nozickova, Heikenwalder, Gores, Weber: "Hepatocyte apoptosis is tumor promoting in murine nonalcoholic steatohepatitis." in: Cell death & disease, Vol. 11, Issue 2, pp. 80, (2021) (PubMed).

Jayavelu, Schnöder, Perner, Herzog, Meiler, Krishnamoorthy, Huber, Mohr, Edelmann-Stephan, Austin, Brandt, Palandri, Schröder, Isermann, Edlich, Sinha, Ungelenk, Hübner, Zeiser, Rahmig, Waskow, Coldham, Ernst, Hochhaus, Jilg, Jost, Mullally, Bullinger, Me: "Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms." in: Nature, Vol. 588, Issue 7836, pp. 157-163, (2021) (PubMed).

Patel, Mendoza, Robichaux, Wang, Wehrens, Karch: "Inhibition of the Anti-Apoptotic Bcl-2 Family by BH3 Mimetics Sensitize the Mitochondrial Permeability Transition Pore Through Bax and Bak." in: Frontiers in cell and developmental biology, Vol. 9, pp. 765973, (2021) (PubMed).

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抗原

MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

别名

Mcl-1 (MCL1 产品)

背景

Synonyms: rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
Background: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.
Gene Name: Mcl1

基因ID

17210

UniProt

P97287

途径

MAPK Pathway