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GLI1 抗体 (AA 805-820)

Name: GLI1 抗体 (AA 805-820)
SKU: ABIN6657595
Availability: OutOfStock

Rockland 100-401-223s GLI1 适用: 小鼠 WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated

Western Blot - Gli1 Antibody Western blot using anti-Gli-1 antibody shows detection of a band at ~150 kDa (arrowhead) corresponding to human Gli-1 present in transfected 293T cell lysates (lanes 2 and 4). (GLI1 抗体 (AA 805-820))

Anti-Gli-1 Antibody - Immunofluorescence. (GLI1 抗体 (AA 805-820))

Anti-Gli-1 Antibody - Western Blot. (GLI1 抗体 (AA 805-820))

3 images

(8 references)

产品编号 ABIN6657595

Supplier Product No.: 100-401-223s

发货至: 中国

Datasheet as PDF

抗原 See all GLI1 抗体

GLI1 (Zinc Finger Protein GLI1 (GLI1))
抗原表位
16

5

4

4

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 805-820
适用
68

38

28

17

4

4

4

4

3

3

2
小鼠
宿主
55

13

3

1
兔
Compatible Secondaries
克隆类型
51

21
多克隆
标记
40

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This GLI1 antibody is un-conjugated
应用范围
52

18

16

14

13

13

8

7

6

3

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.

100-401-223s

Supplier

Rockland

原理

Gli1 Antibody

交叉反应（详细）

Reactivity is observed against Mouse and Human Gli-1.

纯化方法

This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.

过滤

Sterile filtered

免疫原

The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805-820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP).

亚型

IgG

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GLI1 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 1B9F8 unconjugated

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anti-Zinc Finger Protein GLI1 (GLI1) (AA 805-820) antibody
GLI1 适用: 小鼠 WB, ELISA, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated

anti-Zinc Finger Protein GLI1 (GLI1) (AA 401-500) antibody
GLI1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G11 unconjugated

anti-Zinc Finger Protein GLI1 (GLI1) (AA 200-300) antibody
GLI1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Zinc Finger Protein GLI1 (GLI1) (C-Term) antibody
GLI1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Zinc Finger Protein GLI1 (GLI1) (AA 921-1106) antibody
GLI1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Zinc Finger Protein GLI1 (GLI1) (AA 196-230) antibody
GLI1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB54395 unconjugated

anti-Zinc Finger Protein GLI1 (GLI1) (AA 401-500) antibody
GLI1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C9 unconjugated

应用备注

ELISA_Dilution: 1:20,000 - 1:100,000
IF_Microscopy_Dilution: 1:500 - 1:2,000
Western_Blot_Dilution: 1:2,000 - 1:10,000
Other: User Optimized

说明

Suggested Applications: ChIP, IHC, IP
This antibody has been tested for use in ELISA, Immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, perform heat mediated antigen retrieval via the microwave method before commencing with staining.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

有效期

12 months
Takeuchi, Kito, Itoh, Naruse, Fujikawa, Sadek, Akiyama, Yamashiro, Wakisaka, Abe: "Kruppel-Like Factor 4 represses osteoblast differentiation via ciliary Hedgehog signaling." in: Experimental cell research, Vol. 371, Issue 2, pp. 417-425, (2018) (PubMed).

Armas-López, Piña-Sánchez, Arrieta, de Alba, Ortiz-Quintero, Santillán-Doherty, Christiani, Zúñiga, Ávila-Moreno: "Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients." in: Oncotarget, Vol. 8, Issue 40, pp. 67056-67081, (2017) (PubMed).

Yoon, Lamm, Iannaccone, Higashiyama, Leong, Iannaccone, Walterhouse: "p53 modulates the activity of the GLI1 oncogene through interactions with the shared coactivator TAF9." in: DNA repair, Vol. 34, pp. 9-17, (2016) (PubMed).

Kotula, Sun, Li, Pratico, Fereshteh, Ahrens, Sullenger, Kovacs: "Targeted disruption of β-arrestin 2-mediated signaling pathways by aptamer chimeras leads to inhibition of leukemic cell growth." in: PLoS ONE, Vol. 9, Issue 4, pp. e93441, (2015) (PubMed).

Gurung, Feng, Hua: "Menin directly represses Gli1 expression independent of canonical Hedgehog signaling." in: Molecular cancer research : MCR, Vol. 11, Issue 10, pp. 1215-22, (2014) (PubMed).

Jalili, Mertz, Romanov, Wagner, Kalthoff, Stuetz, Pathria, Gschaider, Stingl, Wagner: "NVP-LDE225, a potent and selective SMOOTHENED antagonist reduces melanoma growth in vitro and in vivo." in: PLoS ONE, Vol. 8, Issue 7, pp. e69064, (2013) (PubMed).

Maliniemi, Carlsson, Kaukola, Ovaska, Niiranen, Saksela, Jeskanen, Hautaniemi, Ranki: "NAV3 copy number changes and target genes in basal and squamous cell cancers." in: Experimental dermatology, Vol. 20, Issue 11, pp. 926-31, (2012) (PubMed).

Yoon, Gilbertson, Iannaccone, Iannaccone, Walterhouse: "Defining a role for Sonic hedgehog pathway activation in desmoplastic medulloblastoma by identifying GLI1 target genes." in: International journal of cancer, Vol. 124, Issue 1, pp. 109-19, (2009) (PubMed).
抗原

GLI1 (Zinc Finger Protein GLI1 (GLI1))

别名

Gli1 (GLI1 产品)

背景

Synonyms: rabbit anti-Gli-1 Antibody, rabbit anti-Gli1 Antibody, Zinc finger protein GLI1 antibody, Glioma-associated oncogene antibody, Oncogene GLI antibody
Background: Anti Gli1 Antibody was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region of Gli1 is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places Gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that Gli is expressed in embryonal carcinoma cells but not in most adult tissue. Gli has been localized to 12q13-q14.3 by Southern blot analysis. In mice, the gene is located on chromosome 10. In mice, three zinc finger transcription factors, Gli-1, Gli-2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli-1 expression is central and probably sufficient for tumor development in humans.
Gene Name: GLI1

基因ID

14632, 4885279

UniProt

P47806

途径

Hedgehog Signaling, Dopaminergic Neurogenesis