Cytochrome P450 抗体

Immunogen: Anti-Cytochrome p450 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human cytochrome p450 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Immunohistochemistry Dilution: 1:100 - 1:200

Application Note: Anti-Cytochrome p450 has been tested in Western Blot, Immunofluorescence and Immunohistochemistry. Expect a band at ~58kda in western blot using appropriate lysates. Positive controls used include an overexpressing lysate and PC-12 at 1 μg/mL for WB, human kidney and/or lung for IHC, HepG2 for IF at 15 μg/mL.

ELISA Dilution: 5.0 μg/mL

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 15 μg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Synonyms: Rabbit Anti-Cytochrome p450 Antibody, Rabbit Anti-CYP1A1 Antibody, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1

Background: Cytochrome p450 belongs to an enzyme superfamily that catalyzes many reactions such as the synthesis of cholesterol, lipids, steroids by oxidizing the compounds and plays a role in drug metabolism. The protein has been known to be stimulated by cigarette smoke. On-going studies link the cytochrome p450 protein with lung cancer. In the liver microsome, it is part of the NADPH-dependent electron transport pathway. The protein has been found expressed in the lung, lymphocytes and placenta. Anti-Cytochrome p450 antibody is useful for researchers interested in cancer research and enzyme research.

Gene Name: CYP1A1