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Bassoon 抗体 (N-Term)

BSN 适用: 人 IHC, WB, FM, MA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6657297
发货至: 中国
  • 抗原 See all Bassoon (BSN) 抗体
    Bassoon (BSN)
    抗原表位
    • 13
    • 2
    • 1
    • 1
    N-Term
    适用
    • 24
    • 14
    • 14
    • 1
    • 1
    宿主
    • 24
    • 3
    克隆类型
    • 24
    • 3
    多克隆
    标记
    • 11
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Bassoon antibody is un-conjugated
    应用范围
    • 19
    • 18
    • 4
    • 4
    • 3
    • 2
    Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM), Multiplex Assay (MA)
    原理
    BASSOON Antibody
    交叉反应 (详细)
    A BLAST analysis was used to suggest cross-reactivity with Bassoon from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.
    纯化方法
    Anti-Bassoon Antibody was purified by affinity chromatography.
    过滤
    Sterile filtered
    免疫原
    Bassoon Antibody was produced from whole rabbit serum prepared by repeated immunizations with a N-terminal his-tagged fusion protein.
    亚型
    IgG
    Top Product
    Discover our top product BSN Primary Antibody
  • 应用备注

    Immunohistochemistry_Dilution: User Optimized

    IF_Microscopy_Dilution: 1:400

    Western_Blot_Dilution: 1:1000

    说明

    Anti-Bassoon Antibody is tested for use in WB and IHC. Expect a band approximately ~420kDa on specific lysates corresponding to the molecular mass of Bassoon. Multiple isoforms can be detected. Specific conditions for reactivity should be optimized by the end user. Product provided in PBS pH 7.4.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: See application note.

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.09 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Bassoon (BSN)
    别名
    Bassoon (BSN 产品)
    别名
    Znf231 antibody, ZNF231 antibody, bassoon antibody, bassoon (presynaptic cytomatrix protein) antibody, bassoon presynaptic cytomatrix protein antibody, Bsn antibody, BSN antibody
    背景

    Synonyms: BSN, ZNF231, Neuronal double zine finger protein, protein bassoon

    Background: Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.

    Gene Name: BSN

    基因ID
    8927
    NCBI登录号
    NP_003449
    UniProt
    Q9UPA5
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