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ATM 抗体 (pSer1981) (HRP)

ATM 适用: 人 WB, IHC, ELISA, FM 宿主: 小鼠 Monoclonal 10H11-E12 HRP
产品编号 ABIN6656106
发货至: 中国
  • 抗原 See all ATM 抗体
    ATM (Ataxia Telangiectasia Mutated (ATM))
    抗原表位
    • 28
    • 16
    • 15
    • 13
    • 9
    • 8
    • 7
    • 7
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1974-1988, pSer1981
    适用
    • 148
    • 75
    • 32
    • 1
    • 1
    • 1
    宿主
    • 130
    • 19
    • 5
    • 2
    小鼠
    克隆类型
    • 128
    • 28
    单克隆
    标记
    • 74
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    This ATM antibody is conjugated to HRP
    应用范围
    • 66
    • 46
    • 39
    • 31
    • 28
    • 23
    • 21
    • 18
    • 17
    • 10
    • 8
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)
    交叉反应
    人, 小鼠, 大鼠
    交叉反应 (详细)
    Cross reactivity with ATM from other mammalian sources has not been tested.
    纯化方法
    HRP conjugated ATM phospho S1981 Monoclonal Antibody is Protein A Purified directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human and mouse ATM.
    免疫原

    Immunogen: ATM phospho S1981 Peroxidase Conjugated Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

    Immunogen Type: Peptide

    克隆位点
    10H11-E12
    亚型
    IgG1
    Top Product
    Discover our top product ATM Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: This product has been tested by ELISA, IF, and western blotting against both the native and recombinant forms of the protein. This reagent may also be suitable for immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.

    ELISA Dilution: 1:70,000

    Western Blot Dilution: 1:600

    IF Microscopy Dilution: 1:200

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store Anti-ATM phospho S1981 Peroxidase Conjugated Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This ATM antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
  • 抗原
    ATM (Ataxia Telangiectasia Mutated (ATM))
    别名
    ATM (ATM 产品)
    别名
    ATM antibody, Atm antibody, CG6535 antibody, Dmel\\CG6535 antibody, Tefu antibody, atm antibody, atm/tefu antibody, dATM antibody, tef antibody, Xatm antibody, at1 antibody, atdc antibody, tel1 antibody, telo1 antibody, AT1 antibody, ATA antibody, ATC antibody, ATD antibody, ATDC antibody, ATE antibody, TEL1 antibody, TELO1 antibody, AI256621 antibody, C030026E19Rik antibody, telomere fusion antibody, ATM serine/threonine kinase L homeolog antibody, ATM serine/threonine kinase antibody, ataxia telangiectasia mutated antibody, ataxia telangiectasia mutated (atm) antibody, serine/threonine-protein kinase ATM antibody, tefu antibody, atm.L antibody, atm antibody, ATM antibody, EDI_100660 antibody, CpipJ_CPIJ001772 antibody, BDBG_08252 antibody, PAAG_02532 antibody, MCYG_05088 antibody, VDBG_06833 antibody, ACLA_015700 antibody, LOC5565620 antibody, MGYG_07634 antibody, PGTG_14279 antibody, Atm antibody
    背景

    Synonyms: mouse anti-ATM antibody biotin, biotin conjugated mouse anti-ATMpS1981 antibody, mouse anti-ATM pS1981 antibody biotin conjugaed, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody, AT mutated antibody, AT protein antibody, AT1 antibody, ATA antibody, Ataxia telangiectasia gene mutated in human beings antibody, Ataxia telangiectasia mutated antibody, ATC antibody, ATDC antibody, ATE antibody, ATM antibody

    Background: ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure.  The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.

    Gene Name: ATM

    基因ID
    472
    UniProt
    Q13315
    途径
    p53 Pathway, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
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