我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Hsc70 抗体

Name: Hsc70 抗体
SKU: ABIN6656068
Availability: OutOfStock

Rockland 200-301-a28 HSPA8 适用: 人 WB, IHC, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal N27F3-4 unconjugated

TonEBP deficiency promotes energy expenditure and beiging of WAT. (Hsc70 抗体)

Anti-Hsc70 (Hsp73) Antibody - Western Blot. (Hsc70 抗体)

Adipocyte TonEBP expression is elevated in obesity and TonEBP-deficient mice resist obesity. (Hsc70 抗体)

4 images

(4 references)

产品编号 ABIN6656068

Supplier Product No.: 200-301-a28

发货至: 中国

Datasheet as PDF

抗原 See all Hsc70 (HSPA8) 抗体

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
适用
77

42

38

16

12

9

8

6

6

6

5

5

5

4

4

3

3

2

1

1

1

1

1

1

1
人
宿主
69

26

5

2
小鼠
Compatible Secondaries
克隆类型
67

34
单克隆
标记
56

18

11

3

2

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
应用范围
89

50

37

28

28

26

13

11

11

11

10

5

4

2

2

2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Supplier Product No.

200-301-a28

Supplier

Rockland

原理

Hsc70 (Hsp73) Antibody

交叉反应（详细）

A BLAST analysis was used to suggest cross-reactivity with Hsc70 from human, bovine, mouse, rat, C. Elegans

纯化方法

This Protein G purified monoclonal antibody reacts with human Hsc70 (Hsp73) protein.

过滤

Sterile filtered

免疫原

This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide corresponding to a region of human Hsc70 (Hsp73) protein.

克隆位点

N27F3-4

亚型

IgG1

Top Product

Discover our top product HSPA8 Primary Antibody
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 小鼠 Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (Internal Region) antibody
Verified HSPA8 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

应用备注

ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:200 - 1:1,000
Western_Blot_Dilution: 1:500 - 1:2,000
Other: User Optimized

说明

This Protein G purified antibody has been tested for use in western blotting, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a doublet band approximately 72/73 kDa in size corresponding to Hsc70 (Hsp73) by western blotting in the appropriate cell lysate or extract. In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsc70 (Hsp73) in 20 μg of heat shocked HeLa cell lysate by western blotting.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.1 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

有效期

12 months
Kim, Kim, Lee, Eom, Kim, Park, Jeong, Lee: "Transcription Factor TonEBP Stimulates Hyperosmolality-Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus." in: Frontiers in endocrinology, Vol. 12, pp. 627343, (2021) (PubMed).

Ye, Lee, Yoo, Lee, Lee, Kang, Jeong, Park, Lee-Kwon, Choi, Kwon: "TonEBP in dendritic cells mediates pro-inflammatory maturation and Th1/Th17 responses." in: Cell death & disease, Vol. 11, Issue 6, pp. 421, (2021) (PubMed).

Lee, An, Ye, Lee, Yoo, Jeong, Kang, Alfadda, Lim, Park, Lee-Kwon, Kim, Choi, Kwon: "TonEBP/NFAT5 promotes obesity and insulin resistance by epigenetic suppression of white adipose tissue beiging." in: Nature communications, Vol. 10, Issue 1, pp. 3536, (2019) (PubMed).

Lee, Sanada, An, Ye, Lee, Seo, Lee, Lee-Kwon, Küper, Neuhofer, Choi, Kwon: "LPS-induced NFκB enhanceosome requires TonEBP/NFAT5 without DNA binding." in: Scientific reports, Vol. 6, pp. 24921, (2018) (PubMed).
抗原

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

别名

Hsc70 Hsp73 (HSPA8 产品)

背景

Synonyms: Heat shock cognate protein 71- kDa antibody, Heat shock protein 8 antibody, Heat-shock70-KD protein 10, formerly antibody, HSC54 antibody, HSC71 antibody
Background: Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.
Gene Name: HSPA1A

基因ID

3312

NCBI登录号

NP_006588

UniProt

P11142