-
- 抗原
- VAMP 1,2
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 200-301-e34
- Supplier
- Rockland
- 原理
- Synaptobrevin (VAMP) Antibody
- 交叉反应 （详细）
- Anti-Synaptobrevin (VAMP) Antibody is directed against human synaptobrevin.
- 纯化方法
- Anti-Synaptobrevin was IgM purified by chromatography from cell culture supernatant.
- 免疫原
- Anti-Synaptobrevin (VAMP) Monoclonal Antibody was produced in mouse by repeated immunizations with synaptic immunoprecipitate (crude) from human brain.
- 克隆位点
- SP10
- 亚型
- IgM
-
anti-VAMP 1,2 antibody
适用: 人 IHC, WB 宿主: 小鼠 Monoclonal SP10 unconjugated
-
- 应用备注
-
Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 1:1000
- 说明
-
Anti-Synaptobrevin antibody has been tested in Western Blotting and IHC. Expect a band of approximately 16 kDa in size corresponding to the VAMP proteins in Western blot of rat brain lysate. Anti-Synaptobrevin antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections. Specific conditions for reactivity should be optimized by the end user. Researchers should determine optimal titers for applications that are not stated below.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
-
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- VAMP 1,2
- 别名
- VAMP1/2
- 背景
-
Synonyms: Vesicle-associated membrane protein 1
Background: Synaptobrevin (also VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles. Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer's disease.
Gene Name: VAMP1/2
- 基因ID
- 6843
- UniProt
- P23763
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