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HSP70 1A 抗体 (AA 436-503)

Name: HSP70 1A 抗体 (AA 436-503)
SKU: ABIN6656050
Availability: OutOfStock

Rockland 200-301-a27 HSPA1A 适用: 人 WB, IHC, ELISA, FACS, FM 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated

Anti-Hsp70 Antibody - Western Blot. (HSP70 1A 抗体 (AA 436-503))

Hsp70 Mouse Melanoma Cells Immunohistochemistry of Mouse anti-Hsp70 monoclonal antibody. (HSP70 1A 抗体 (AA 436-503))

IHC

2 images

(3 references)

产品编号 ABIN6656050

Supplier Product No.: 200-301-a27

发货至: 中国

Datasheet as PDF

抗原 See all HSP70 1A (HSPA1A) 抗体

HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
抗原表位
12

6

6

5

4

4

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 436-503
适用
103

32

24

20

15

10

10

9

7

7

6

6

5

4

4

4

3

3

3

1

1

1

1

1

1
人
宿主
79

42

1
小鼠
Compatible Secondaries
克隆类型
79

43
单克隆
标记
70

13

12

4

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This HSP70 1A antibody is un-conjugated
应用范围
103

48

41

36

31

18

16

10

8

5

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.

200-301-a27

Supplier

Rockland

原理

Hsp70 Antibody

交叉反应（详细）

A BLAST analysis was used to suggest cross-reactivity with Hsp70 from human, bovine, mouse, rat, C.

纯化方法

This Protein G purified monoclonal antibody reacts with human and mouse Hsp70 protein.

过滤

Sterile filtered

免疫原

Hsp70 monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide from the region of amino acid residues 436-503 of human Hsp70 protein.

克隆位点

C92F3A-5

亚型

IgG1

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anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 285-641) antibody
HSPA1A 适用: 人 WB 宿主: 小鼠 Monoclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (C-Term) antibody
HSPA1A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibody
HSPA1A 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibody
HSPA1A 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 2# unconjugated

应用备注

Immunohistochemistry_Dilution: 10 μg/mL
IF_Microscopy_Dilution: 1:200-1:1000
Western_Blot_Dilution: 1:500-1:2000
Other: User Optimized

说明

Suggested Applications: Other
This Protein G purified antibody has been tested for use in ELISA, immunofluorescence microscopy, western blotting, FACS, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 70 kDa in size corresponding to Hsp70 by western blotting in the appropriate cell lysate or extract. In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsp70 in 20 μg of heat shocked HeLa cell lysate by western blotting.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.1 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Hsp70 antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

有效期

12 months
Satoh, Stalder, McKercher, Williamson, Roth, Lipton: "Nrf2 and HSF-1 Pathway Activation via Hydroquinone-Based Proelectrophilic Small Molecules is Regulated by Electrochemical Oxidation Potential." in: ASN neuro, Vol. 7, Issue 4, (2016) (PubMed).

Ristic, Tsou, Guzi, Kanack, Scaglione, Todi: "USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila." in: The Journal of biological chemistry, Vol. 291, Issue 17, pp. 9161-72, (2016) (PubMed).

Satoh, Rezaie, Seki, Sunico, Tabuchi, Kitagawa, Yanagitai, Senzaki, Kosegawa, Taira, McKercher, Hoffman, Roth, Lipton: "Dual neuroprotective pathways of a pro-electrophilic compound via HSF-1-activated heat-shock proteins and Nrf2-activated phase 2 antioxidant response enzymes." in: Journal of neurochemistry, Vol. 119, Issue 3, pp. 569-78, (2011) (PubMed).
抗原

HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

别名

HSPA1A (HSPA1A 产品)

背景

Synonyms: Heat shock 70 kDa protein 1 antibody, heat shock 70 kDa protein 1A antibody, Heat shock 70 kDa protein 1B antibody, Heat shock induced protein antibody, heat shock protein 70 antibody, HSP70 1 antibody
Background: Anti-Hsp 70 antibody is routinely used in cancer and signal transduction research. Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.
Gene Name: HSPA1A

基因ID

3304

NCBI登录号

NP_005336

UniProt

P0DMV8

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process