SMC1B 抗体 (pSer957)
(3 references)-
- 抗原 See all SMC1B 抗体
- SMC1B (Structural Maintenance of Chromosomes 1B (SMC1B))
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抗原表位
- pSer957
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SMC1B antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- 原理
- SMC1 phospho S957 Antibody
- 交叉反应 （详细）
- This monoclonal anti-SMC1 antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.
- 纯化方法
- This Protein G Purified Mab antibody is directed against human SMC1 and is useful in determining its presence in various assays.
- 过滤
- Sterile filtered
- 免疫原
- This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.
- 克隆位点
- 5D11-G5
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product SMC1B Primary Antibody
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anti-Structural Maintenance of Chromosomes 1B (SMC1B) (N-Term) antibody
SMC1B 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Structural Maintenance of Chromosomes 1B (SMC1B) (AA 954-1060) antibodySMC1B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Structural Maintenance of Chromosomes 1B (SMC1B) (AA 551-660) antibodySMC1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6A10 unconjugated
anti-Structural Maintenance of Chromosomes 1B (SMC1B) antibodySMC1B 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Structural Maintenance of Chromosomes 1B (SMC1B) (AA 737-765) antibodySMC1B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33824 unconjugated
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- 应用备注
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ELISA_Dilution: 1:20,000 - 1:100,000
Immunohistochemistry_Dilution: 2.5 μg/mL
IF_Microscopy_Dilution: 2.5 μg/mL
Western_Blot_Dilution: 1:100 - 1:2,000
Other: User Optimized
- 说明
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Suggested Applications: IF, Multiplex
Protein G Purified Mab anti-SMC1 was tested by ELISA, immunohistochemistry and western blotting against native protein. The antibody reacts with SMC1 from irradiated human and mouse cells. A 160 kDa band corresponding to phosphorylated human SMC1 is noted in gamma irradiated human and mouse lysates. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Essential role for DNA-PKcs in DNA double-strand break repair and apoptosis in ATM-deficient lymphocytes." in: Molecular cell, Vol. 34, Issue 3, pp. 285-97, (2009) (PubMed).
: "ATM promotes apoptosis and suppresses tumorigenesis in response to Myc." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1446-51, (2006) (PubMed).
: "Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway." in: Genes & development, Vol. 18, Issue 12, pp. 1423-38, (2004) (PubMed).
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: "Essential role for DNA-PKcs in DNA double-strand break repair and apoptosis in ATM-deficient lymphocytes." in: Molecular cell, Vol. 34, Issue 3, pp. 285-97, (2009) (PubMed).
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- 抗原
- SMC1B (Structural Maintenance of Chromosomes 1B (SMC1B))
- 别名
- SMC1B (SMC1B 产品)
- 别名
- SMC1BETA antibody, SMC1L2 antibody, bK268H5 antibody, bK268H5.5 antibody, SMC-1B antibody, SMC1beta antibody, Smc1l2 antibody, structural maintenance of chromosomes 1B antibody, SMC1B antibody, Smc1b antibody
- 背景
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Synonyms: mouse anti-SMC1 pS957 antibody, Structural maintenance of chromosomes protein 1B antibody, SMC1beta protein antibody, SMC1B antibody, SMC1L2 antibody
Background: Structural maintenance of chromosomes (SMC) proteins play important roles in sister chromatid cohesion, chromosome condensation, sex-chromosome dosage compensation, and DNA recombination and repair (DNA damage). Protein complexes containing heterodimers of the SMC1 and SMC3 proteins have been implicated specifically in both sister chromatid cohesion and DNA recombination. ATM, a protein kinase belonging to the phosphatidylinositol 3-kinase family that regulates cell cycle checkpoints and DNA recombination and repair, phosphorylates SMC1 protein after ionizing irradiation. ATM protein kinase phosphorylates SMC1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint. Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM protein kinase substrates NBS1 and BRCA1. These same sites in SMC1 are phosphorylated after treatment with UV irradiation or hydroxyurea in an ATM-independent manner, thus demonstrating that another kinase must be involved in responses to these cellular stresses. Yeast containing hypomorphic mutations in SMC1 and human cells overexpressing SMC1 mutated at both of these phosphorylation sites exhibit decreased survival following ionizing irradiation. These results demonstrate that SMC1 participates in cellular responses to DNA damage and link SMC1 to the ATM protein kinase signal transduction pathway.
Gene Name: SMC1B
- 基因ID
- 27127
- UniProt
- Q8NDV3
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