PD-L1 抗体
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- 抗原 See all PD-L1 抗体
- PD-L1 (CD274 (PD-L1))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PD-L1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-101-gu8
- Supplier
- Rockland
- 原理
- PDL1 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against human PDL1.
- 纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
- Anti-PDL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human PDL1 conjugated to Keyhole Limpet Hemocyanin (KLH).
- 亚型
- IgG
- Top Product
- Discover our top product PD-L1 Primary Antibody
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anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 9B5F4 unconjugated
anti-CD274 (PD-L1) (AA 39-191) antibodyPD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 6D9A2 unconjugated
anti-CD274 (PD-L1) (AA 48-290) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (Extracellular Domain) antibodyPD-L1 适用: 人 IHC 宿主: 兔 Monoclonal unconjugated
anti-CD274 (PD-L1) (AA 34-241) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (C-Term) antibodyKO Validated PD-L1 适用: 人 WB, FACS, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7D2A10 unconjugated
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- 应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: 1:10,000-1:50,000
Immunohistochemistry_Dilution: 1:200
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: 10 μg/mL
Western_Blot_Dilution: 1.0 μg/mL
- 说明
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Anti-PDL-1 Antibody has been tested in Western Blot, ELISA, and IF. Positive control whole cell lysates used MDA-MB-231 p/n (W09-001-GK6) and A549 p/n (W09-001-372) and positive recombinant protein control for western blotting. Expect a band at ~33.3 kDa in western blot using appropriate lysates. Immunofluorescence positive control cell line used was MDA-MB-231 p/n (W09-001-GK6). Although not tested, this antibody is likely functional in flow cytometry and immunoprecipitation.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- PD-L1 (CD274 (PD-L1))
- 别名
- PDL1 (PD-L1 产品)
- 背景
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Synonyms: Goat anti-PDL1 antibody, Programmed cell death 1 ligand 1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, PD-L1, PDCD1 ligand 1, B7-H1, B7 homolog 1, PDL-1, CD274 Molecule antibody, MGC142294 antibody, PDL 1
Background: PDL1 belongs to the immunoglobulin superfamily and BTN/MOG family. PDL1 is involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFNG, in an IL-2 dependent and a PDCD1 independent manner. It is up-regulated in T-and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. PDL1 is up-regulated in B-cells activated by surface Ig cross-linking. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Anti-PDL-1 Antibody is useful for researches interested in cancer research, NF-kB pathway research, and the Immune system.
Gene Name: CD274
- 基因ID
- 29126
- NCBI登录号
- NP_001254635
- UniProt
- Q9NZQ7
- 途径
- Cancer Immune Checkpoints
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