我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

ATM 抗体 (AA 1974-1988)

Name: ATM 抗体 (AA 1974-1988)
SKU: ABIN6655681
Availability: OutOfStock

ATM 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7C10D8 unconjugated

Determination of ATM and ATMp antibodies specificity by western blotting. (ATM 抗体 (AA 1974-1988))

Anti-ATM Monoclonal Antibody - Immunohistochemistry Anti ATM monoclonal Antibody was used to show constitutive activation of the ATM pathway in human urinary bladder cancer. (ATM 抗体 (AA 1974-1988))

2 images

(16 references)

产品编号 ABIN6655681

发货至: 中国

Datasheet as PDF

抗原 See all ATM 抗体

ATM (Ataxia Telangiectasia Mutated (ATM))
抗原表位
29

16

15

13

9

8

7

7

7

5

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1974-1988
适用
148

75

32

1

1

1
人
宿主
130

19

5

2
小鼠
Compatible Secondaries
克隆类型
128

28
单克隆
标记
73

9

7

5

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

2

2
This ATM antibody is un-conjugated
应用范围
66

46

39

31

28

23

20

18

17

10

8

7

4

2

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

交叉反应

人, 小鼠

纯化方法

This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence by immunohistochemistry. This monoclonal anti-ATM antibody recognizes the phosphorylated form of the protein in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human and mouse ATM. Cross reactivity with ATM from other mammalian sources has not been tested.

免疫原

Immunogen: This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
Immunogen Type: Peptide

克隆位点

7C10D8

亚型

IgG2a

Top Product

Discover our top product ATM Primary Antibody
anti-Ataxia Telangiectasia Mutated (ATM) (AA 1974-1988), (pSer1981) antibody
ATM 适用: 人 WB, IHC, ELISA, FACS, FM 宿主: 小鼠 Monoclonal 10H11-E12 unconjugated

anti-Ataxia Telangiectasia Mutated (ATM) (AA 1974-1988), (pSer1981) antibody
ATM 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, FM 宿主: 小鼠 Monoclonal 10H11-E12 unconjugated

anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody
ATM 适用: 人, 小鼠 WB, IHC, ELISA, IP, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ataxia Telangiectasia Mutated (ATM) (AA 2681-2750) antibody
ATM 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Ataxia Telangiectasia Mutated (ATM) (AA 2484-2748) antibody
ATM 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ataxia Telangiectasia Mutated (ATM) antibody
ATM 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Monoclonal 4E11 unconjugated Recombinant Antibody

anti-Ataxia Telangiectasia Mutated (ATM) (AA 1974-1988), (pSer1981) antibody (HRP)
ATM 适用: 人 WB, IHC, ELISA, FM 宿主: 小鼠 Monoclonal 10H11-E12 HRP

anti-Ataxia Telangiectasia Mutated (ATM) (AA 486-742) antibody
ATM 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ataxia Telangiectasia Mutated (ATM) antibody
ATM 适用: 人 IHC, ELISA, FACS 宿主: 兔 Monoclonal 3G11 unconjugated Recombinant Antibody

anti-Ataxia Telangiectasia Mutated (ATM) antibody
ATM 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C5 unconjugated

应用备注

Immunohistochemistry Dilution: 1:100 - 1:500
Application Note: This Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody clone has been optimized for IHC, but may also be used for western blotting, flow cytometry, immunoprecipitation, or immunofluorescence microscopy. Indirect immunoperoxidase staining on formaldehyde-fixed, de-paraffinized tissue sections and/or formalin-fixed cultured cells are recommended when using this antibody as described in Bartkova et al 2005. Product p/n 200-301-400 produced from clone 10H11.E12 has been optimized for WB, IP and IF.
ELISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: User Optimized
Western Blot Dilution: 1:500- 1:2,000

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium Azide
Stabilizer: None

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

RT,4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Rosina, Langone, Giuliani, Cerquone Perpetuini, Reggio, Calderone, Fuoco, Castagnoli, Gargioli, Cesareni: "Osteogenic differentiation of skeletal muscle progenitor cells is activated by the DNA damage response." in: Scientific reports, Vol. 9, Issue 1, pp. 5447, (2019) (PubMed).

Emery, Gopalan, Wood, Chow, Battelli, George, Blaszyk, Florman, Yun: "Expression and function of ABCG2 and XIAP in glioblastomas." in: Journal of neuro-oncology, Vol. 133, Issue 1, pp. 47-57, (2018) (PubMed).

Sinha, Qin, Li: "A p53/ARF-dependent anticancer barrier activates senescence and blocks tumorigenesis without impacting apoptosis." in: Molecular cancer research : MCR, Vol. 13, Issue 2, pp. 231-8, (2016) (PubMed).

Liu, Xu, OPrey, Lao, Joshi, Long, OPrey, Croft, Beaumatin, Baudot, Mrschtik, Rosenfeldt, Zhang, Gillespie, Ryan: "Loss of autophagy causes a synthetic lethal deficiency in DNA repair." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 3, pp. 773-8, (2015) (PubMed).

Phesse, Myant, Cole, Ridgway, Pearson, Muncan, van den Brink, Vousden, Sears, Vassilev, Clarke, Sansom: "Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo." in: Cell death and differentiation, Vol. 21, Issue 6, pp. 956-66, (2014) (PubMed).

Broering, Alavattam, Sadreyev, Ichijima, Kato, Hasegawa, Camerini-Otero, Lee, Andreassen, Namekawa: "BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis." in: The Journal of cell biology, Vol. 205, Issue 5, pp. 663-75, (2014) (PubMed).

Shamma, Suzuki, Hayashi, Kobayashi, Sasaki, Nishiuchi, Doki, Okamoto, Kohno, Muranaka, Kitajima, Yamamoto, Takahashi: "ATM mediates pRB function to control DNMT1 protein stability and DNA methylation." in: Molecular and cellular biology, Vol. 33, Issue 16, pp. 3113-24, (2013) (PubMed).

Parikh, Shuck, Nguyen, Herron, Donehower: "Mouse tissues that undergo neoplastic progression after K-Ras activation are distinguished by nuclear translocation of phospho-Erk1/2 and robust tumor suppressor responses." in: Molecular cancer research : MCR, Vol. 10, Issue 6, pp. 845-55, (2012) (PubMed).

Schwab, Smith, Dressler: "Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes." in: Developmental biology, Vol. 373, Issue 1, pp. 64-71, (2012) (PubMed).

Lantuejoul, Raynaud, Salameire, Gazzeri, Moro-Sibilot, Soria, Brambilla, Brambilla: "Telomere maintenance and DNA damage responses during lung carcinogenesis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 11, pp. 2979-88, (2010) (PubMed).

Navaraj, Finnberg, Dicker, Yang, Matthew, El-Deiry: "Reduced cell death, invasive and angiogenic features conferred by BRCA1-deficiency in mammary epithelial cells transformed with H-Ras." in: Cancer biology & therapy, Vol. 8, Issue 24, pp. 2417-44, (2010) (PubMed).

Leemput, Masson, Bigot, Errachid, Dansault, Provost, Gadin, Aoufouchi, Menasche, Abitbol: "ATM localization and gene expression in the adult mouse eye." in: Molecular vision, Vol. 15, pp. 393-416, (2009) (PubMed).

Raynaud, Jang, Nuciforo, Lantuejoul, Brambilla, Mounier, Olaussen, André, Morat, Sabatier, Soria: "Telomere shortening is correlated with the DNA damage response and telomeric protein down-regulation in colorectal preneoplastic lesions." in: Annals of oncology : official journal of the European Society for Medical Oncology, Vol. 19, Issue 11, pp. 1875-81, (2008) (PubMed).

Bartkova, Bakkenist, Rajpert-De Meyts, Skakkebaek, Sehested, Lukas, Kastan, Bartek: "ATM activation in normal human tissues and testicular cancer." in: Cell cycle (Georgetown, Tex.), Vol. 4, Issue 6, pp. 838-45, (2006) (PubMed).

Kitagawa, Bakkenist, McKinnon, Kastan: "Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway." in: Genes & development, Vol. 18, Issue 12, pp. 1423-38, (2004) (PubMed).

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, (2003) (PubMed).
抗原

ATM (Ataxia Telangiectasia Mutated (ATM))

别名

ATM (ATM 产品)

别名

ATM antibody, Atm antibody, CG6535 antibody, Dmel\\CG6535 antibody, Tefu antibody, atm antibody, atm/tefu antibody, dATM antibody, tef antibody, Xatm antibody, at1 antibody, atdc antibody, tel1 antibody, telo1 antibody, AT1 antibody, ATA antibody, ATC antibody, ATD antibody, ATDC antibody, ATE antibody, TEL1 antibody, TELO1 antibody, AI256621 antibody, C030026E19Rik antibody, telomere fusion antibody, ATM serine/threonine kinase L homeolog antibody, ATM serine/threonine kinase antibody, ataxia telangiectasia mutated antibody, ataxia telangiectasia mutated (atm) antibody, serine/threonine-protein kinase ATM antibody, tefu antibody, atm.L antibody, atm antibody, ATM antibody, EDI_100660 antibody, CpipJ_CPIJ001772 antibody, BDBG_08252 antibody, PAAG_02532 antibody, MCYG_05088 antibody, VDBG_06833 antibody, ACLA_015700 antibody, LOC5565620 antibody, MGYG_07634 antibody, PGTG_14279 antibody, Atm antibody

背景

Synonyms: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody
Background: Anti ATM pS1981 Antibody recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. See Bakkenist, C. J. & Kastan, M. B. Nature 421, 499-506 (2003) for a complete presentation of this antibody's specificity and utility.
Gene Name: ATM

基因ID

472

UniProt

Q13315

途径

p53 Pathway, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus