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HIST1H3A 抗体 (H3K27me)

Rockland 600-401-w54 HIST1H3A 适用: 人 ELISA, WB, IF, ChIP, DB, FM 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN6655652
Supplier Product No.: 600-401-w54
发货至: 中国
  • 抗原 See all HIST1H3A 抗体
    HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
    抗原表位
    • 16
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
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    • 4
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    • 2
    • 1
    • 1
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    H3K27me
    适用
    • 194
    • 60
    • 48
    • 16
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 190
    • 4
    克隆类型
    • 156
    • 38
    多克隆
    标记
    • 170
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    This HIST1H3A antibody is un-conjugated
    应用范围
    • 154
    • 128
    • 74
    • 62
    • 52
    • 44
    • 28
    • 20
    • 13
    • 13
    • 10
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    ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-w54
    Supplier
    Rockland
    原理
    Histone H3 K27me1 Antibody
    交叉反应 (详细)
    This product reacts with human Histone 3 methyl Lys27.
    纯化方法
    Anti-Histone H3 k27 me1 Antibody was purified by affinity purification.
    过滤
    Sterile filtered
    免疫原
    Anti-Histone H3 K27 me1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of histone H3 containing the monomethylated lysine 27.
    亚型
    IgG
    Top Product
    Discover our top product HIST1H3A Primary Antibody
  • 应用备注

    ChIP_Dilution: 1 μg per IP

    ELISA_Dilution: 1:500

    IF_Microscopy_Dilution: 1:1,000

    Western_Blot_Dilution: 1:1,000

    Other: Dot Blot: 1:20,000

    说明

    Anti-Histone H3 K27 me1 Antibody is tested in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15kDa in the appropriate cell lysate or extract.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300
    储存液
    ProClin, Sodium azide
    注意事项
    This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
    别名
    HIST1H3A (HIST1H3A 产品)
    背景

    Synonyms: Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/e, Histone H3/f, Histone H3/g, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

    Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called ""histone code"". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Anti-Histone H3 K27 me1 Antibody is ideal for research in Chromatin Remodeling and Genetics.

    Gene Name: HIST1H3A

    基因ID
    3020
    NCBI登录号
    NP_002098
    UniProt
    P84243
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