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PLK1 抗体

Rockland 200-901-mh7 PLK1 适用: 人 WB, ELISA, IHC, IF, FM 宿主: 小鸡 Polyclonal unconjugated
Rockland
产品编号 ABIN6655434
Supplier Product No.: 200-901-mh7
发货至: 中国
  • 抗原 See all PLK1 抗体
    PLK1 (Polo-Like Kinase 1 (PLK1))
    适用
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    小鸡
    克隆类型
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    多克隆
    标记
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    This PLK1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)
    Supplier Product No.
    200-901-mh7
    Supplier
    Rockland
    原理
    PLK1 Antibody
    交叉反应 (详细)
    Reactivity occurs against human PLK1.
    纯化方法
    This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
    过滤
    Sterile filtered
    免疫原
    Anti-PLK1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to an internal portion of human PLK1 conjugated to Keyhole Limpet Hemocyanin (KLH).
    亚型
    IgG
    Top Product
    Discover our top product PLK1 Primary Antibody
  • 应用备注

    ELISA_Dilution: 1:10,000 - 1:50,000

    Immunohistochemistry_Dilution: 15 μg/mL

    IF_Microscopy_Dilution: 5 μg/mL

    Western_Blot_Dilution: 1:500

    说明

    Anti-PLK1 antibody has been tested in Western Blot and Immunofluorescence. Expect a band ~68.3kDa in appropriate cell lysates or tissues. Positive controls used are HEK293T or K-562 whole cell lysate in WB, and HeLa cells in IF. Specific conditions for reactivity should be optimized by the end user.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    PLK1 (Polo-Like Kinase 1 (PLK1))
    别名
    PLK1 (PLK1 产品)
    背景

    Synonyms: Chicken Anti-Polo Like Kinase 1 Antibody, Chicken Anti-PLK1 Antibody, Chicken PLK-1 Antibody, Serine/Threonine-Protein Kinase 13, Polo-Like Kinase 1, STPK13, PLK-1, PLK, Serine/Threonine-Protein Kinase PLK1, Cell Cycle Regulated Protein Kinase, Polo-Like Kinase (Drosophila), Polo (Drosophila)-Like Kinase, EC 2.7.11.21

    Background: Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that belongs to the CDC5/Polo subfamily and are critical regulators of cell cycle progression, mitosis, cytokinesis, elevated levels are found in many different types of cancer, and the DNA damage response. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis, hence, it is a target for cancer therapy. PLK-1, -2 and -3 are ubiquitously expressed, whereas PLK-4 is restricted to a few tissues. Diseases associated with PLK1 include Breast Cancer, Transient Tic Disorder, and Monoclonal Paraproteinemia. Anti-PLK1 Antibody is useful for researchers interested in Cancer Researcher, Cell Cycle Research, and Kinase Activity Research.

    Gene Name: PLK1

    基因ID
    5347
    NCBI登录号
    NP_005021
    UniProt
    P53350
    途径
    Cell Division Cycle, M Phase
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