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POLR2A/RPB1 抗体 (pSer5)

Rockland 200-301-v01 POLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated

Western Blot of anti-Pol II S5p antibody Western Blot results of Mouse anti-Pol II S5p antibody. (POLR2A/RPB1 抗体 (pSer5))

Chromatin Immunoprecipitation was performed on Pol II S5p antibody Chromatin Immunoprecipitation was performed on sheared chromatin from 1 million HeLaS3 cells using 1 μg of Pol II S5p antibody. (POLR2A/RPB1 抗体 (pSer5))

Chromatin Immunoprecipitation on Pol II S5p antibody Chromatin Immunoprecipitation was performed on sheared chromatin from 1 million HeLaS3 cells using 1 μg of Pol II S5p antibody. (POLR2A/RPB1 抗体 (pSer5))

Chromatin Immunoprecipitation Anti-Pol-II-S5p- Chromatin Immunoprecipitation was performed on sheared chromatin from 1 million HeLaS3 cells using 1 μg of Pol II S5p antibody. (POLR2A/RPB1 抗体 (pSer5))

ChIP on Pol II S5p antibody Chromatin Immunoprecipitation was performed on sheared chromatin from 1 million HeLaS3 cells using 1 μg of Pol II S5p antibody. (POLR2A/RPB1 抗体 (pSer5))

Chromatin immunoprecipitation for Anti-Pol II S5p Antibody Chromatin immunoprecipitation assays were performed using sheared chromatin from 1 million HeLa cells for Anti-Pol II S5p Antibody and optimized PCR primer pairs for qPCR. (POLR2A/RPB1 抗体 (pSer5))

Immunofluorescence Microscopy of anti-Pol II S5p antibody Immunofluorescence Microscopy results of Mouse anti-Pol II S5p antibody. (POLR2A/RPB1 抗体 (pSer5))

7 images

产品编号 ABIN6655367

Supplier Product No.: 200-301-v01

发货至: 中国

Datasheet as PDF

抗原 See all POLR2A/RPB1 (POLR2A) 抗体

POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
抗原表位
17

16

11

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer5
适用
85

40

39

6

6

6

5

5

4

3

3

2

2

1

1
人
宿主
69

16

1

1

1
小鼠
Compatible Secondaries
克隆类型
49

39
单克隆
标记
57

5

4

4

4

4

4

4

1

1
This POLR2A/RPB1 antibody is un-conjugated
应用范围
51

31

25

25

15

10

9

7

4

3

3

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Supplier Product No.

200-301-v01

Supplier

Rockland

原理

POL II phospho S5 Antibody

交叉反应（详细）

This antibody is specific for Pol II protein phosphorylated Ser5.

纯化方法

Anti-Pol II pS5 Antibody was purified by Protein A chromatography.

过滤

Sterile filtered

免疫原

Anti-Pol II S5p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser5 of the repeated sequence.

亚型

IgG1

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anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibody
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anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibody
POLR2A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibody
POLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibody
POLR2A 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Monoclonal 2G1 unconjugated Recombinant Antibody

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 128-258) antibody
POLR2A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1B1D8 unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 76-298) antibody
POLR2A 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibody
POLR2A 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 2H4 unconjugated Recombinant Antibody

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibody
POLR2A 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal CTD4H8 unconjugated

anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibody
POLR2A 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal CTD 8A7 unconjugated

应用备注

ChIP_Dilution: 1 μg/ChIP
ELISA_Dilution: 1:3,000
IF_Microscopy_Dilution: 1:500
Western_Blot_Dilution: 1:1,000

说明

Anti-Pol II pS5 Antibody is tested for Chromatin Immunoprecipitation Sequencing, Chromatin Immunoprecipitation, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 225 kDa in the appropriate cell lysate or extract.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

别名

Pol II (POLR2A 产品)

背景

Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S5p Antibody is ideal for research in Gene Expression, Transcription and Genetics.
Gene Name: POLR2A

基因ID

5430

NCBI登录号

NP_000928

UniProt

P24928

途径

Regulatory RNA Pathways

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