POLR2A/RPB1 抗体 (pSer5)
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- 抗原 See all POLR2A/RPB1 (POLR2A) 抗体
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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抗原表位
- pSer5
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This POLR2A/RPB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
- 纯化方法
- Anti-Pol II pS5 Antibody was purified by Protein A chromatography. This antibody is specific for Pol II protein phosphorylated Ser5. Cross-reactivity with Pol II pS5 from other sources has not been determined.
- 免疫原
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Immunogen: Anti-Pol II S5p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser5 of the repeated sequence.
Immunogen Type: Peptide
- 亚型
- IgG1
- Top Product
- Discover our top product POLR2A Primary Antibody
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anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibody
POLR2A 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, ELISA, ChIP, FM, CUT&RUN 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Monoclonal 2G1 unconjugated Recombinant Antibody
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 128-258) antibodyPOLR2A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1B1D8 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 76-298) antibodyPOLR2A 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Monoclonal 2H4 unconjugated Recombinant Antibody
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal CTD4H8 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal CTD 8A7 unconjugated
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- 应用备注
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The rabbit anti-PolII (pSer5) antibody ABIN6655367 is suitable for use in ChIP, CUT&RUN, ELISA, immunofluorescence microscopy, and western blot. Specific conditions for each assay should be optimized by the end user. Expect a band of approximately 225 kDa in western blot in the appropriate cell lysate or extract.
General ABIN6655367 dilution recommendations for different applications are as follows:
- ChIP: 1 µg/ChIP
- ELISA: 1:3,000
- IF: 1:500
- WB: 1:1,000
- CUT&RUN: 1:100
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02 % (w/v) Sodium AzideStabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- 抗原
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
- 别名
- Pol II (POLR2A 产品)
- 别名
- NCU02103.1 antibody, 19.m02399 antibody, POLR2A antibody, 220kDa antibody, Rpb1 antibody, Rpo2-1 antibody, POLR2 antibody, POLRA antibody, RPB1 antibody, RPBh1 antibody, RPO2 antibody, RPOL2 antibody, RpIILS antibody, hRPB220 antibody, hsRPB1 antibody, DNA-directed RNA polymerase II largest subunit antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II large subunit Rpb1 antibody, RNA polymerase II subunit A antibody, DNA-directed RNA polymerase II largest subunit, putative antibody, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody, polymerase (RNA) II (DNA directed) polypeptide A antibody, NCU02103 antibody, BBOV_I003360 antibody, EDI_169920 antibody, CMU_000800 antibody, SJAG_01713 antibody, MGYG_01527 antibody, POLR2A antibody, TP03_0185 antibody, EBI_22514 antibody, CGB_E5020W antibody, polr2a antibody, Polr2a antibody
- 背景
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Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S5p Antibody is ideal for research in Gene Expression, Transcription and Genetics.
Gene Name: POLR2A
- 基因ID
- 5430
- NCBI登录号
- NP_000928
- UniProt
- P24928
- 途径
- Regulatory RNA Pathways
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