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POLR2A/RPB1 抗体 (pSer2)

POLR2A 适用: 人 WB, ELISA, IF, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN6655366
发货至: 中国
  • 抗原 See all POLR2A/RPB1 (POLR2A) 抗体
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    抗原表位
    • 17
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    pSer2
    适用
    • 81
    • 40
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    • 6
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    宿主
    • 65
    • 16
    • 1
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    小鼠
    克隆类型
    • 49
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    单克隆
    标记
    • 53
    • 5
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    This POLR2A/RPB1 antibody is un-conjugated
    应用范围
    • 47
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    Western Blotting (WB), ELISA, Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
    原理
    POL II phospho S2 Antibody
    交叉反应 (详细)
    This antibody is specific for Pol II protein phosphorylated Ser2.
    纯化方法
    Anti-Pol II pS2 Antibody was purified by Protein A chromatography.
    过滤
    Sterile filtered
    免疫原
    Anti-Pol II S2p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser2 of the repeated sequence.
    亚型
    IgG1
    Top Product
    Discover our top product POLR2A Primary Antibody
  • 应用备注

    ChIP_Dilution: 1 μg/ChIP

    ELISA_Dilution: 1:3,000

    IF_Microscopy_Dilution: 1:500

    Western_Blot_Dilution: 1:1,000

    说明

    Anti-Pol II pS2 Antibody is tested for Chromatin Immunoprecipitation Sequencing, Chromatin Immunoprecipitation, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 225 kDa in the appropriate cell lysate or extract.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.05 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    别名
    Pol II (POLR2A 产品)
    别名
    NCU02103.1 antibody, 19.m02399 antibody, POLR2A antibody, 220kDa antibody, Rpb1 antibody, Rpo2-1 antibody, POLR2 antibody, POLRA antibody, RPB1 antibody, RPBh1 antibody, RPO2 antibody, RPOL2 antibody, RpIILS antibody, hRPB220 antibody, hsRPB1 antibody, DNA-directed RNA polymerase II largest subunit antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II large subunit Rpb1 antibody, RNA polymerase II subunit A antibody, DNA-directed RNA polymerase II largest subunit, putative antibody, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody, polymerase (RNA) II (DNA directed) polypeptide A antibody, NCU02103 antibody, BBOV_I003360 antibody, EDI_169920 antibody, CMU_000800 antibody, SJAG_01713 antibody, MGYG_01527 antibody, POLR2A antibody, TP03_0185 antibody, EBI_22514 antibody, CGB_E5020W antibody, polr2a antibody, Polr2a antibody
    背景

    Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

    Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S2p Antibody is ideal for research in Gene Expression, Transcription, and Genetics.

    Gene Name: POLR2A

    基因ID
    5430
    NCBI登录号
    NP_000928
    UniProt
    P24928
    途径
    Regulatory RNA Pathways
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