TLR3 抗体
-
- 抗原 See all TLR3 抗体
- TLR3 (Toll-Like Receptor 3 (TLR3))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This TLR3 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
- Supplier Product No.
- 200-301-i24
- Supplier
- Rockland
- 原理
- TLR3 Antibody
- 交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with Anti-TLR3 from dog, human and mouse based on 100 % homology with the immunizing sequence.
- 纯化方法
- Anti-TLR3 Antibody was purified by Protein G chromatography.
- 免疫原
- TLR3 Antibody was produced in mice prepared by repeated immunizations with a peptide corresponding to internal amino acid sequences in the human protein TLR3.
- 克隆位点
- 40C1285-6
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TLR3 Primary Antibody
-
-
- 应用备注
-
Immunoprecipitation_Dilution: 2 μg/10^6 cells
Immunohistochemistry_Dilution: 10 μg/mL
Flow_Cytometry_Dilution: 2-4 μg/10^6 cells
Western_Blot_Dilution: 1-3 μg/mL
- 说明
-
Anti-TLR3 Antibody is tested for use in WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, and Bioactivity. Expect a band approximately 120kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- TLR3 (Toll-Like Receptor 3 (TLR3))
- 别名
- TLR3 (TLR3 产品)
- 背景
-
Synonyms: Toll-like receptor 3, CD283
Background: Anti-TLR3 Antibody detects human TLR3. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. TLR3 cDNA codes for a protein with approximate molecular weight of 120 kDa. TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes . The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. Anti-TLR3 Antibody is ideal for investigators involved in cytokines and growth factor research.
Gene Name: TLR3
- 基因ID
- 7098
- NCBI登录号
- NP_003256
- UniProt
- O15455
- 途径
- TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
-