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CD20 抗体 (APC)

Rockland 200-326-n64 MS4A1 适用: 人 FACS, IHC, IP 宿主: 小鼠 Monoclonal 2H7 APC
Rockland
产品编号 ABIN6655312
Supplier Product No.: 200-326-n64
发货至: 中国
  • 抗原 See all CD20 (MS4A1) 抗体
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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    小鼠
    克隆类型
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    单克隆
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    This CD20 antibody is conjugated to APC
    应用范围
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    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Supplier Product No.
    200-326-n64
    Supplier
    Rockland
    原理
    CD20 Allophycocyanin Antibody
    交叉反应 (详细)
    Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey.
    纯化方法
    Allophycocyanin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20.
    过滤
    Sterile filtered
    免疫原
    Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.
    克隆位点
    2H7
    亚型
    IgG2b kappa
    Labeling Ratio
    1-2
    Top Product
    Discover our top product MS4A1 Primary Antibody
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

    说明

    Anti-CD20 is tested for Flow Cytometry and useful in Immunoprecipitation and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.2 % BSA (w/v)

    Preservative: 0.09 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
    有效期
    6 months
  • Tedder, Engel: "CD20: a regulator of cell-cycle progression of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 450-4, (1994) (PubMed).

    Hultin, Hausner, Hultin, Giorgi: "CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes." in: Cytometry, Vol. 14, Issue 2, pp. 196-204, (1993) (PubMed).

  • 抗原
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    别名
    CD20 (MS4A1 产品)
    背景

    Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20

    Background: CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 Molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

    Gene Name: MS4A1

    基因ID
    931
    NCBI登录号
    NP_068769
    UniProt
    P11836
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