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TERT 抗体 (C-Term)

Name: TERT 抗体 (C-Term)
SKU: ABIN6655122
Availability: OutOfStock

TERT 适用: 人 WB, ELISA, IHC, IF, IP, FM 宿主: 兔 Polyclonal unconjugated

Rabbit anti-hTERT WB Western Blot of Rabbit anti-Telomerase catalytic subunit antibody. (TERT 抗体 (C-Term))

anti-hTERT antibody-Immunofluorescence. (TERT 抗体 (C-Term))

Anti-hTERT Antibody - Immunofluorescence Microscopy anti hTERT antibody-Immunofluorescence# anti hTERT antibody was used to stain hTERT on hTERT-over-expressing fibroblasts. (TERT 抗体 (C-Term))

Anti-hTERT Antibody - Immunofluorescence Microscopy Immunofluorescence microscopy of Saos-2 cells transduced with a retroviral vector expressing hTERT and green fluorescent protein (GFP) from an internal ribosomal entry site (IRES). (TERT 抗体 (C-Term))

Anti-hTERT Antibody - Immunoprecipitation/Western Blot Immunoprecipitation of hTERT protein from NIH 3T3 cell lysates. (TERT 抗体 (C-Term))

Anti-hTERT Antibody - Western Blot. (TERT 抗体 (C-Term))

6 images

(5 references)

产品编号 ABIN6655122

发货至: 中国

Datasheet as PDF

抗原 See all TERT 抗体

TERT (Telomerase Reverse Transcriptase (TERT))
抗原表位
16

16

10

10

6

5

5

4

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
107

37

20

5

1

1
人
宿主
110

5

1
兔
Compatible Secondaries
克隆类型
102

14
多克隆
标记
55

8

7

6

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This TERT antibody is un-conjugated
应用范围
70

53

38

27

17

14

14

13

7

7

4

4

3

3

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

原理

hTERT Antibody

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

纯化方法

Affinity Purified Anti-hTERT Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads.

过滤

Sterile filtered

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of hTERT (accession number AF018167).

亚型

IgG

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TERT 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Telomerase Reverse Transcriptase (TERT) (pTyr707) antibody
TERT 适用: 人 ELISA, IF, DB 宿主: 兔 Polyclonal unconjugated

应用备注

Immunoprecipitation_Dilution: IP 2μL per mg lysate
ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:500
IF_Microscopy_Dilution: 1:500
Western_Blot_Dilution: 1:500

说明

Suggested Applications: ChIP, FISH, IHC, Multiplex
Anti-Telomerase catalytic subunit antibody has been tested for use in ELISA, immunoblotting, immunoprecipitation, and immunofluorescence microscopy. In these assays, the antibody detects ectopically-expressed hTERT and high levels of endogenous hTERT. A SY5Y cell nuclear extract can be used as a positive control. This antibody primarily detects hTERT, but several non-specific bands appear on immunoblots. In immunofluorescence microscopy assays, staining with anti-TERT-16 was specific to the nuclei of cells with ectopic TERT expression. In immunoblot assays, whole cell or nuclear extracts were loaded at a concentration of 100 μg protein per well. A working dilution of 1:500 anti-TERT antibody was used followed by a 1:3,000 dilution of HRP goat anti-rabbit IgG as the secondary antibody. For immunofluorescence microscopy staining, a working dilution of 1:500 was used followed by a 1:200 dilution of rhodamine-conjugated donkey anti-rabbit IgG as a secondary antibody. Immunoprecipitation was performed using 20 μL of protein A beads and 2 μL of the anti-TERT serum per 1mg protein from cell lysate. A working dilution of 1:500 is also suggested for immunohistochemistry. To detect TERT, fix cells in 2% paraformaldehyde (in PBS) for 10'. Wash the slides twice in PBS for 5' each. Permeabilize the cells in 0.5% NP-40 for 10'. Wash as before in PBS. Block the cells using PBG buffer (0.2% cold water fish gelatin (Sigma G-7765) and 0.5% BSA in PBS) for 20' at room temperature. Incubate in primary antibody (diluted in PBG) for 1-2 hours at RT or overnight at 4°C. Wash the slides three times in PBG for 5' each. Incubate with secondary antibody (diluted in PBG) for 1 hour at RT in the dark. Wash the slides three times in PBG for 5' each. Mount in DAPI-containing medium.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

有效期

3 months
Bernal, Zafon, Domínguez, Bertran, Tusell: "Generation of Immortalised But Unstable Cells after hTERT Introduction in Telomere-Compromised and p53-Deficient vHMECs." in: International journal of molecular sciences, Vol. 19, Issue 7, (2018) (PubMed).

Eitan, Braverman, Tichon, Gitler, Hutchison, Mattson, Priel: "Excitotoxic and Radiation Stress Increase TERT Levels in the Mitochondria and Cytosol of Cerebellar Purkinje Neurons." in: Cerebellum (London, England), Vol. 15, Issue 4, pp. 509-17, (2017) (PubMed).

Radan, Hughes, Teichroeb, Vieira Zamora, Jewer, Postovit, Betts: "Microenvironmental regulation of telomerase isoforms in human embryonic stem cells." in: Stem cells and development, Vol. 23, Issue 17, pp. 2046-66, (2014) (PubMed).

Theurillat, Udeshi, Errington, Svinkina, Baca, Pop, Wild, Blattner, Groner, Rubin, Moch, Privé, Carr, Garraway: "Prostate cancer. Ubiquitylome analysis identifies dysregulation of effector substrates in SPOP-mutant prostate cancer." in: Science (New York, N.Y.), Vol. 346, Issue 6205, pp. 85-9, (2014) (PubMed).

Wu, Dudognon, Nguyen, Hillion, Pendino, Tarkanyi, Aradi, Lanotte, Tong, Chen, Ségal-Bendirdjian: "Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths." in: Journal of cell science, Vol. 119, Issue Pt 13, pp. 2797-806, (2006) (PubMed).
抗原

TERT (Telomerase Reverse Transcriptase (TERT))

别名

TERT (TERT 产品)

别名

CMM9 antibody, DKCA2 antibody, DKCB4 antibody, EST2 antibody, PFBMFT1 antibody, TCS1 antibody, TP2 antibody, TRT antibody, hEST2 antibody, hTRT antibody, chTERT antibody, Tert antibody, ATTERT antibody, F5E19.190 antibody, F5E19_190 antibody, telomerase reverse transcriptase antibody, tert antibody, TR antibody, est2 antibody, tcs1 antibody, telomerase antibody, tert-A antibody, tp2 antibody, trt antibody, xTERT antibody, telomerase reverse transcriptase antibody, Telomerase Reverse Transcriptase antibody, Tertp antibody, Telomerase catalytic subunit antibody, telomerase reverse transcriptase L homeolog antibody, TERT antibody, trt-1 antibody, Tert antibody, tert antibody, CMU_003490 antibody, VDBG_04487 antibody, GL50803_16225 antibody, tert.L antibody

背景

Synonyms: rabbit anti-TERT antibody, rabbit anti-Telomerase catalytic subunit antibody, hTERT, Telomerase reverse transcriptase, HEST2, Telomerase-associated protein 2, TP2, EST2, TCS1, TRT
Background: Telomerase is a reverse transcriptase that adds telomeric repeats (TTAGGG)n to chromosomal ends, compensating for the telomere shortening that occurs with DNA replication. In normal human somatic cells, telomerase is repressed and telomeres progressively shorten, leading to limited lifespan and senescence. Reactivation of telomerase activity is associated with human cancer and cell immortalization. Approximately 85 % of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity, whereas most normal somatic cells do not. The specificity of telomerase to human cancer has led to investigations of telomerase activity and expression as a tumor marker. For example, the presence of telomerase activity in human urine has been identified as a marker for human bladder carcinoma. Human telomerase consists of three major subunits: a catalytic protein subunit called hTERT (for human Telomerase Reverse Transcriptase), a template RNA called hTR, and telomerase-associated protein (TEP-1). TERT and hTR are minimally required to reconstitute telomerase activity in vitro. In human cells, hTR is constitutively expressed. TERT transcription is a primary mechanism for regulation of telomerase activity.
Gene Name: TERT

基因ID

7015

NCBI登录号

NP_001180305

UniProt

O14746

途径

Telomere Maintenance