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- 抗原 See all CD25 (IL2RA) 抗体
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD25 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 交叉反应 (详细)
- Cross reactivity with CD25 from other sources has not been tested
- 纯化方法
- Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25. Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque. . Anti-CD25 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
- 免疫原
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Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.
- 克隆位点
- BC96
- 亚型
- IgG1
- Labeling Ratio
- 4-6
- Top Product
- Discover our top product IL2RA Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-CD25 is useful for Flow Cytometry and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.
Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
0.09 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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- 抗原
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 别名
- CD25 (IL2RA 产品)
- 别名
- CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
- 背景
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Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55
Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Gene Name: IL2RA
- 基因ID
- 3559
- NCBI登录号
- NP_000408
- UniProt
- P01589
- 途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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