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CD25 抗体 (FITC)

Rockland 200-302-n25 IL2RA 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal BC96 FITC
Rockland
产品编号 ABIN6655112
Supplier Product No.: 200-302-n25
发货至: 中国
  • 抗原 See all CD25 (IL2RA) 抗体
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD25 antibody is conjugated to FITC
    应用范围
    • 220
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    Flow Cytometry (FACS), Immunohistochemistry (IHC)
    Supplier Product No.
    200-302-n25
    Supplier
    Rockland
    原理
    CD25 Fluorescein Antibody
    交叉反应 (详细)
    Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque.
    纯化方法
    Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25.
    过滤
    Sterile filtered
    免疫原
    Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.
    克隆位点
    BC96
    亚型
    IgG1 kappa
    Labeling Ratio
    4-6
    Top Product
    Discover our top product IL2RA Primary Antibody
  • 应用备注

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

    说明

    Anti-CD25 is tested for Flow Cytometry and is useful for Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.2 % BSA (w/v)

    Preservative: 0.09 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
    有效期
    6 months
  • Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

    Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).

  • 抗原
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    别名
    CD25 (IL2RA 产品)
    背景

    Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

    Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

    Gene Name: IL2RA

    基因ID
    3559
    NCBI登录号
    NP_000408
    UniProt
    P01589
    途径
    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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