Calbindin-D 抗体
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- 抗原 See all Calbindin-D products
- Calbindin-D
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Calbindin-D antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 交叉反应 （详细）
- Cross reactivity with Calbindin-D from other sources has not been determined.
- 纯化方法
- Anti-Calbindin-D antibody was purified from mouse ascites by protein A chromatography. This product reacts with human, mouse, bovine, and rat.
- 免疫原
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Immunogen: Calbindin-D antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to bovine kidney calbindin-D.
Immunogen Type: Recombinant Protein
- 克隆位点
- CB-D7
- 亚型
- IgG1
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- 应用备注
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Anti-Calbindin-D is useful for Immunohistochemistry -P and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~30 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:10,000
Western Blot Dilution: 0.5-1 μg/mL
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Neutral PBS
- 缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- 抗原
- Calbindin-D
- Abstract
- Calbindin-D 产品
- 背景
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Synonyms: CALB, D-28K, CALBINDIN, 28-KD, Calbindin D28, Vitamin D-dependent calcium-binding protein, avian-type
Background: The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization. This antibody is suitable for researchers interested in transcription factors, nuclear receptors, cancer research, and nutritional and metobolic diseases.
Gene Name: CALB1
- 基因ID
- 793
- NCBI登录号
- NP_004920
- UniProt
- P05937
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