Ubiquitin B 抗体
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- 抗原 See all Ubiquitin B (UBB) 抗体
- Ubiquitin B (UBB)
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适用
- Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Ubiquitin B antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Supplier Product No.
- 200-301-g61
- Supplier
- Rockland
- 原理
- Ubiquitin Antibody
- 交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with ubiquitin from Human, mouse, rat, and bovine based on 100 % homology with the immunizing sequence.
- 纯化方法
- Anti-Ubiquitin Antibody was purified by Protein G chromatography.
- 过滤
- Sterile filtered
- 免疫原
- Ubiquitin Antibody was produced in mice by repeated immunizations raised against native bovine ubiquitin conjugated to KLH.
- 克隆位点
- 5B9-B3
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product UBB Primary Antibody
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anti-Ubiquitin B (UBB) (AA 1-119) antibody
UBB 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal UBB-1748 unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB 适用: 人 WB, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal UBB-2122 unconjugated
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3C12 unconjugated
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-76) antibodyUBB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Ubiquitin B (UBB) (AA 1-76) antibodyUBB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 77-152) antibodyUBB 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 103-228) antibodyUBB 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB 适用: 人 IHC, StM 宿主: 兔 Monoclonal UBB-3143R unconjugated Recombinant Antibody
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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ELISA_Dilution: 1:200
Western_Blot_Dilution: 1:1000
- 说明
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Anti-Ubiquitin Antibody is tested for use in WB and IF. Expect a band approximately ~10kDa corresponding to free ubiquitin. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Ubiquitin B (UBB)
- 别名
- UBB (UBB 产品)
- 背景
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Synonyms: Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B
Background: Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity. The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate. Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBα and p27.
Gene Name: UBB
- 基因ID
- 281370
- NCBI登录号
- NP_776558
- UniProt
- P0CG53
- 途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway
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