SPTA1 抗体 (AA 356-475)
SPTA1
适用: 人
IHC (p), WB
宿主: 小鼠
Monoclonal
SCNA-1R
unconjugated
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- 抗原 See all SPTA1 products
- SPTA1 (Spectrin alpha Chain, Erythrocytic 1 (SPTA1))
- 抗原表位
- AA 356-475
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SPTA1 antibody is un-conjugated
- 应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- 纯化方法
- Purified
- 纯度
- Protein G affinity chromatography
- 免疫原
- A portion of amino acids 356-475 from the human protein was used as the immunogen for the recombinant SPTA1 antibody.
- 克隆位点
- SCNA-1R
- 亚型
- IgG1 kappa
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- 应用备注
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The stated application concentrations are suggested starting points. Titration of the recombinant SPTA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) - 限制
- 仅限研究用
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- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the recombinant SPTA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- SPTA1 (Spectrin alpha Chain, Erythrocytic 1 (SPTA1))
- 别名
- SPTA1 / Spectrin alpha 1 (SPTA1 产品)
- 背景
- This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [RefSeq]
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