RLTPR 抗体 (PE)
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- 抗原 See all RLTPR products
- RLTPR (RGD Motif, Leucine Rich Repeats, Tropomodulin Domain and Proline-Rich Containing (RLTPR))
- 适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This RLTPR antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.
- 交叉反应 (详细)
- Human, Mouse
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- Murine RLTPR
- 克隆位点
- EM-53
- 亚型
- IgG1 kappa
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- 应用备注
- Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- 抗原
- RLTPR (RGD Motif, Leucine Rich Repeats, Tropomodulin Domain and Proline-Rich Containing (RLTPR))
- 别名
- RLTPR / CARMIL2 (RLTPR 产品)
- 别名
- CG1399-PB antibody, D130029J02Rik antibody, Gm585 antibody, CARMIL2 antibody, CARMIL2b antibody, LRRC16C antibody, capping protein regulator and myosin 1 linker 2 antibody, F-actin-uncapping protein RLTPR antibody, RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing antibody, Carmil2 antibody, CARMIL2 antibody, LOC100021093 antibody, LOC100091608 antibody, RLTPR antibody
- 背景
- Capping protein regulator and myosin 1 linker 2,RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing, capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 Molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.,CARMIL2, LRRC16C
- 基因ID
- 146206
- UniProt
- Q6F5E8
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