Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
预测反应
B, Pr
纯化方法
This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the N-terminal region of human PRMT5.
HRMT1L5 antibody, IBP72 antibody, JBP1 antibody, SKB1 antibody, SKB1Hs antibody, Jbp1 antibody, Skb1 antibody, si:zc14a17.2 antibody, skb1 antibody, wu:fb95f10 antibody, zgc:110669 antibody, hsl7 antibody, DDBDRAFT_0187422 antibody, DDBDRAFT_0235403 antibody, DDB_0187422 antibody, DDB_0235403 antibody, NV18830 antibody, jbp1 antibody, ibp72 antibody, skb1hs antibody, hrmt1l5 antibody, protein arginine methyltransferase 5 antibody, protein arginine N-methyltransferase 5 antibody, protein arginine methyltransferase 5 L homeolog antibody, putative arginine N-methyltransferase, type II antibody, Skb1 family protein antibody, Protein arginine N-methyltransferase 5 antibody, PRMT5 antibody, Prmt5 antibody, prmt5 antibody, prmt5.L antibody, LOC100115766 antibody, prmt-5 antibody
背景
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.