Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
预测反应
Zf, B, M, Rb, Rat, X
纯化方法
This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the Central region of human PPP4C.
Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).