Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
预测反应
X
纯化方法
This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 amino acids from the N-terminal region of human SF3B1.
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].