This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This PLOD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 amino acids from the N-terminal region of human PLOD3.
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.