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MITF 抗体 (N-Term)

Name: MITF 抗体 (N-Term)
SKU: ABIN655506
Availability: OutOfStock

MITF 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18810 unconjugated

Anti-MITF Antibody (N-term)at 1:2000 dilution + whole cell lysates Lysates/proteins at 20 μg per lane. (MITF 抗体 (N-Term))

MITF Antibody (N-term) (ABIN655506 and ABIN2845022) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). (MITF 抗体 (N-Term))

FACS

Confocal immunofluorescent analysis of MITF Antibody (N-term) (ABIN655506 and ABIN2845022) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (MITF 抗体 (N-Term))

MITF Antibody (N-term) (ABIN655506 and ABIN2845022) western blot analysis in K562 cell line lysates (35 μg/lane). (MITF 抗体 (N-Term))

Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1 A. (MITF 抗体 (N-Term))

IHC

5 images

(1 reference)

产品编号 ABIN655506

发货至: 中国

Datasheet as PDF

抗原 See all MITF 抗体

MITF (Microphthalmia-Associated Transcription Factor (MITF))
抗原表位
18

15

13

9

7

7

7

7

4

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 1-28, N-Term
适用
116

64

25

17

5

5

5

5

4

4

4

3

2

2

2

2
人
宿主
90

40

1

1
兔
Compatible Secondaries
克隆类型
83

49
多克隆
标记
63

10

4

4

4

4

4

4

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This MITF antibody is un-conjugated
应用范围
69

48

42

37

35

17

13

7

7

3

3

3

2

2

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

预测反应

M, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This MITF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human MITF.

克隆位点

RB18810

亚型

Ig Fraction

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anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 351-450) antibody
MITF 适用: 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer180), (pSer73) antibody
MITF 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) antibody
MITF 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibody
MITF 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-114) antibody
MITF 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3A2E2 unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) antibody
MITF 适用: 人, 犬 IHC, IF, FACS, StM 宿主: 兔 Monoclonal MITF-2987R unconjugated Recombinant Antibody

anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-280) antibody
MITF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibody
MITF 适用: 人, 小鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) antibody
MITF 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal D5 unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-520) antibody
MITF 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. WB: 1:2000. IHC: 1:800. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Ishii, Ryu, Suzuki: "Lactoferrin inhibits melanogenesis by down-regulating MITF in melanoma cells and normal melanocytes." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 95, Issue 1, pp. 119-125, (2017) (PubMed).
抗原

MITF (Microphthalmia-Associated Transcription Factor (MITF))

别名

MITF (MITF 产品)

别名

MITF antibody, ws2a antibody, mitfa antibody, MITF-A antibody, LOC100313655 antibody, mitfb antibody, CMM8 antibody, MI antibody, WS2 antibody, WS2A antibody, bHLHe32 antibody, BCC2 antibody, Bhlhe32 antibody, Gsfbcc2 antibody, Vitiligo antibody, Wh antibody, bw antibody, mi antibody, vit antibody, MITF-H antibody, MITF-M antibody, CMI9 antibody, melanogenesis associated transcription factor antibody, microphthalmia-associated transcription factor antibody, microphthalmia-associated transcription factor S homeolog antibody, Microphthalmia antibody, MITF antibody, mitf antibody, LOC100313655 antibody, mitf.S antibody, LOC100534525 antibody, MICROP antibody, Mitf antibody

背景

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

分子量

58795

基因ID

4286

NCBI登录号

NP_000239, NP_001171896, NP_006713, NP_937801, NP_937802, NP_937820, NP_937821

UniProt

O75030

途径

Chromatin Binding