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SP1 抗体 (C-Term)

Name: SP1 抗体 (C-Term)
SKU: ABIN655398
Availability: OutOfStock

SP1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB17350 unconjugated

Anti-SP1 Antibody (C-term ) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. (SP1 抗体 (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). (SP1 抗体 (C-Term))

FACS

Confocal immunofluorescent analysis of SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (SP1 抗体 (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) western blot analysis in K562 cell line lysates (35 μg/lane). (SP1 抗体 (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. (SP1 抗体 (C-Term))

IHC (p)

5 images

(1 reference)

产品编号 ABIN655398

发货至: 中国

Datasheet as PDF

抗原 See all SP1 抗体

SP1 (Sp1 Transcription Factor (SP1))
抗原表位
21

12

10

6

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 677-707, C-Term
适用
88

60

39

11

8

7

6

5

5

5

4

2

2

2

1
人
宿主
90

13

3
兔
Compatible Secondaries
克隆类型
83

23
多克隆
标记
72

4

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This SP1 antibody is un-conjugated
应用范围
91

46

45

37

21

17

14

13

11

8

4

3

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

预测反应

M, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1.

克隆位点

RB17350

亚型

Ig Fraction

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anti-Sp1 Transcription Factor (SP1) (AA 89-198) antibody
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anti-Sp1 Transcription Factor (SP1) antibody
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anti-Sp1 Transcription Factor (SP1) (C-Term) antibody
SP1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (pThr739) antibody
SP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 89-199) antibody
SP1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4H6 unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 420-620) antibody
SP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 677-707) antibody
SP1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:500. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Zhang, Wang, Qin, Liu, Sun, Liu, Kuang, Jiang, Zhang: "cis-Acting elements and trans-acting factors in the transcriptional regulation of raf kinase inhibitory protein expression." in: PLoS ONE, Vol. 8, Issue 12, pp. e83097, (2014) (PubMed).
抗原

SP1 (Sp1 Transcription Factor (SP1))

别名

SP1 (SP1 产品)

背景

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

分子量

80693

基因ID

6667

NCBI登录号

NP_001238754, NP_003100, NP_612482

UniProt

P08047

途径

Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction