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SP1 抗体 (C-Term)

Name: SP1 抗体 (C-Term)
SKU: ABIN655398
Availability: OutOfStock

SP1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB17350 unconjugated

Anti-SP1 Antibody (C-term ) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. (SP1 抗体 (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). (SP1 抗体 (C-Term))

FACS

Confocal immunofluorescent analysis of SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (SP1 抗体 (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) western blot analysis in K562 cell line lysates (35 μg/lane). (SP1 抗体 (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. (SP1 抗体 (C-Term))

IHC (p)

5 images

(1 reference)

产品编号 ABIN655398

发货至: 中国

Datasheet as PDF

抗原 See all SP1 抗体

SP1 (Sp1 Transcription Factor (SP1))
抗原表位
21

14

12

7

7

6

6

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 677-707, C-Term
适用
97

67

46

11

8

7

6

5

5

5

4

2

2

2

1
人
宿主
99

13

3
兔
Compatible Secondaries
克隆类型
94

21
多克隆
标记
70

6

3

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This SP1 antibody is un-conjugated
应用范围
93

49

47

40

20

15

14

14

13

7

3

3

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

预测反应

M, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1.

克隆位点

RB17350

亚型

Ig Fraction

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anti-Sp1 Transcription Factor (SP1) (C-Term) antibody
SP1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

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SP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

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anti-Sp1 Transcription Factor (SP1) (AA 420-620) antibody
SP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 677-707) antibody
SP1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:500. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Zhang, Wang, Qin, Liu, Sun, Liu, Kuang, Jiang, Zhang: "cis-Acting elements and trans-acting factors in the transcriptional regulation of raf kinase inhibitory protein expression." in: PLoS ONE, Vol. 8, Issue 12, pp. e83097, (2014) (PubMed).
抗原

SP1 (Sp1 Transcription Factor (SP1))

别名

SP1 (SP1 产品)

别名

fc18h09 antibody, zgc:85950 antibody, wu:fc18h09 antibody, SP1 antibody, 1110003E12Rik antibody, AA450830 antibody, AI845540 antibody, Sp1-1 antibody, sp1 transcription factor antibody, Sp1 transcription factor antibody, Sp1 transcription factor L homeolog antibody, trans-acting transcription factor 1 antibody, sp1 antibody, SP1 antibody, Sp1 antibody, sp1.L antibody

背景

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

分子量

80693

基因ID

6667

NCBI登录号

NP_001238754, NP_003100, NP_612482

UniProt

P08047

途径

Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction